Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Mediator | stimulates the CTD kinase activity of TFIIH manyfold, immobilized holo-RNAPII can be separated into RNAPII and Mediator | Saccharomyces cerevisiae | |
additional information | mediator-induced stimulation of C-terminal domain phosphorylation with TFIIH and CTDK1 kinase | Saccharomyces cerevisiae | |
Rsp5 | stimulates CTD kinase activity | Saccharomyces cerevisiae | |
Rsp5 protein | the unrelated CTD-binding protein, Rsp5, is capable of stimulating this CTD kinase activity | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | immobilization of holo-RNAPII via Rbp2 inhibits CTD hyperphosphorylation | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNA polymerase II C-terminal repeat domain | Saccharomyces cerevisiae | hyperphosphorylation, the Mediator complex associates with RNA polymerase II, RNAPII, at least partly via the RNAPII C-terminal repeat domain, CTD, whose phosphorylation is involved in triggering promoter clearance | ADP + phosphoylated RNA polymerase II C-terminal repeat domain | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native TFIIK, and native CTD kinase 1 by a tandem affinity purification method | Saccharomyces cerevisiae |
TFIIK, CTDK1 by tandem affinity purification | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + holo-RNA polymerase II | C-terminal domain phosphorylation by the serine 5-specific TFIIH complex, its kinase module TFIIK or by the C-terminal domain serine 2-specific kinase CTDK1 | Saccharomyces cerevisiae | ? | - |
? | |
ATP + RNA polymerase II C-terminal repeat domain | hyperphosphorylation, the Mediator complex associates with RNA polymerase II, RNAPII, at least partly via the RNAPII C-terminal repeat domain, CTD, whose phosphorylation is involved in triggering promoter clearance | Saccharomyces cerevisiae | ADP + phosphoylated RNA polymerase II C-terminal repeat domain | - |
? | |
ATP + RNA polymerase II C-terminal repeat domain | i.e. RNAPII CTD, hyperphosphorylation, the target specificity of TFIIK and CTDK1 differm, TFIIK phosphorylates Ser5, while CTDK1 phosphorylates Ser2 | Saccharomyces cerevisiae | ADP + phosphoylated RNA polymerase II C-terminal repeat domain | - |
? | |
additional information | enzyme-Mediator complex dissociation assay, overview | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CTD kinase | - |
Saccharomyces cerevisiae |
CTDK1 | - |
Saccharomyces cerevisiae |
CTDK1 kinase | - |
Saccharomyces cerevisiae |
TFIIH | - |
Saccharomyces cerevisiae |
TFIIK | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae | |
ATP | ATP-dependent dissociation of holo-RNAPII by TFIIH | Saccharomyces cerevisiae |