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Literature summary for 2.7.11.23 extracted from
- Phosphorylation of the C-terminal domain of RNA polymerase II plays central roles in the integrated events of eucaryotic gene expression (2007), J. Biochem., 141, 601-608.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | required for accurate transcription initiation and transcription elongation | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | loss of Ser2 phosphorylation through inactivation of the CTD kinase Cdk9 leads to a defect in processing but not in transcription elongation | Drosophila melanogaster |
| additional information | loss of Ser2 phosphorylation through inactivation of the CTD kinase Ctk1 in yeast leads to a defect in processing but not in transcription elongation, crosslinking of 3' end processing factors to the 3' end region of genes is dramatically decreased | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| flavopiridol | causes a reduction in Ser2 phosphorylation and a defect in the transcription elongation of heat shock genes | Saccharomyces cerevisiae |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + RNA polymerase II C-terminal subunit | Homo sapiens | the TFIIH mediator complex plays essential roles in transcription initiation and during the transition from initiation to elongation by transmitting signals from transcriptional activators to RNA polymerase II, phosphorylation of the C-terminal domain of RNA polymerase II plays central roles in the integrated events of eucaryotic gene expression, it is not only essential for transcription, but also as a platform for RNA processing and chromatin regulation, detailed overview | ADP + phosphoylated RNA polymerase II C-terminal subunit | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
- |
- |
| Homo sapiens | - |
isozymes TFIIH, TFIIF, and TFIIE | - |
| Saccharomyces cerevisiae | - |
- |
- |
| Xenopus laevis | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| oocyte | - |
Xenopus laevis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + RNA polymerase II | C-terminal domain phosphorylation is required for co-transcriptional splicing | Xenopus laevis | ? | - |
? | |
| ATP + RNA polymerase II | phosphorylation on C-terminal domain | Drosophila melanogaster | ? | - |
? | |
| ATP + RNA polymerase II | TFIIH and mediator phosphorylate at C-terminal domain at Ser 5, Ser5 phosphorylation by the yeast Cdk7 (Kin28) subunit of TFIIH is required for recruitment of capping enzyme to the promoter region, P-TEFb phosphorylates at Ser 2 | Saccharomyces cerevisiae | ? | - |
? | |
| ATP + RNA polymerase II C-terminal subunit | the TFIIH mediator complex plays essential roles in transcription initiation and during the transition from initiation to elongation by transmitting signals from transcriptional activators to RNA polymerase II, phosphorylation of the C-terminal domain of RNA polymerase II plays central roles in the integrated events of eucaryotic gene expression, it is not only essential for transcription, but also as a platform for RNA processing and chromatin regulation, detailed overview | Homo sapiens | ADP + phosphoylated RNA polymerase II C-terminal subunit | - |
? | |
| ATP + RNA polymerase II C-terminal subunit | i.e. Pol II CTD, TFIIH and mediator phosphorylate Ser5 of the CTD heptapeptide repeat sequence | Homo sapiens | ADP + phosphoylated RNA polymerase II C-terminal subunit | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the three CTD kinase isozymes possess CDK-cyclin pairs, and TFIIH additionally forms a preinitiation complex, overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDK9 | - |
Drosophila melanogaster |
| CTD kinase | - |
Drosophila melanogaster |
| CTD kinase | - |
Homo sapiens |
| CTD kinase | - |
Saccharomyces cerevisiae |
| CTD kinase | - |
Xenopus laevis |
| Ctk1 | - |
Saccharomyces cerevisiae |
| P-TEFb | - |
Saccharomyces cerevisiae |
| TFIIH | - |
Homo sapiens |
| TFIIH | - |
Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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