Literature summary for 2.7.11.23 extracted from

Jones, J.C.; Phatnani, H.P.; Haystead, T.A.; MacDonald, J.A.; Alam, S.M.; Greenleaf, A.L.
C-terminal repeat domain kinase I phosphorylates Ser2 and Ser5 of RNA polymerase II C-terminal domain repeats (2004), J. Biol. Chem., 279, 24957-24964.

Search for more literature for 2.7.11.23

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
nucleus	-	Saccharomyces cerevisiae	5634	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + [DNA-directed RNA polymerase]	Saccharomyces cerevisiae	-	ADP + phospho-[DNA-directed RNA polymerase]	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	gene ctk1	-

Purification (Commentary)

Purification (Comment)	Organism
native CTDK-I	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + [DNA-directed RNA polymerase]	-	Saccharomyces cerevisiae	ADP + phospho-[DNA-directed RNA polymerase]	-	?
ATP + [DNA-directed RNA polymerase]	recombinant GST-tagged protein substrate or synthetic GST-tagged peptide substrate derived from RNA polymerase II, CTD kinase 1 hyperphosphorylates the C-terminal repeat domain CTD of the RNA polymerase II large subunit at Ser2 and/or Ser5, determination of phosphorylation sites, already phosphorylated substrates are more efficient substrates for CTD kinase I	Saccharomyces cerevisiae	ADP + phospho-[DNA-directed RNA polymerase]	-	?

Synonyms

Synonyms	Comment	Organism
C-terminal repeat domain kinase I	-	Saccharomyces cerevisiae
CTDK-I	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.6	-	assay at	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)