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Literature summary for 2.7.11.23 extracted from
- 5,6-Dichloro-1-beta-D-ribofuranosylbenzimidazole inhibits a HeLa protein kinase that phosphorylates an RNA polymerase II-derived peptide (1989), Biochem. Biophys. Res. Commun., 159, 508-515.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole | nucleotide analog | Homo sapiens |  |
| additional information | no inhibition by EGTA | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
ATP | 27°C, pH 7.5 | Homo sapiens | |
| 0.15 | - |
Synthetic peptide | 27°C, pH 7.5 | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Homo sapiens | |
| Mg2+ | 10 mM | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] | Homo sapiens | - |
ADP + phospho-[DNA-directed RNA polymerase II subunit IIa] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Homo sapiens | - |
| HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + synthetic peptide | - |
Homo sapiens | ADP + phosphorylated synthetic peptide | - |
? | |
| ATP + synthetic peptides | e.g. Lys-(Tyr-Ser-Pro-Thr-Ser-Pro-Ser)4 | Homo sapiens | ADP + ? | - |
? | |
| ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] | - |
Homo sapiens | ADP + phospho-[DNA-directed RNA polymerase II subunit IIa] | - |
? | |
| ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] | phosphorylates predominantly Ser-residues | Homo sapiens | ADP + phospho-[DNA-directed RNA polymerase II subunit IIa] | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | 28 | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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