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Literature summary for 2.7.11.23 extracted from
- Purification of protein kinases that phosphorylate the repetitive carboxyl-terminal domain of eukaryotic RNA polymerase II (1991), Methods Enzymol., 200, 301-325.
General Stability
| General Stability | Organism |
|---|---|
| purified mouse enzymes are rather unstable with a half-life of several days, bovine serum albumin does not stabilize | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole | nucleotide analog | Homo sapiens |  |
| 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole | nucleotide analog | Mus musculus | |
| High salt concentrations | above 250 mM | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
ATP | - |
Homo sapiens | |
| 0.03 | - |
ATP | 30°C, pH 7.9 | Mus musculus | |
| 0.18 | - |
GTP | 30°C, pH 7.9 | Mus musculus | |
| 0.189 | - |
hepta-six peptide | 30°C, pH 7.9 | Mus musculus | |
| 0.2 | - |
hepta-six peptide | 30°C, pH 7.9 | Mus musculus | |
| 0.212 | - |
L-Arg-hepta peptide | 30°C, pH 7.9 | Mus musculus | |
| 0.243 | - |
L-Arg-hepta peptide | 30°C, pH 7.9 | Mus musculus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Mus musculus | |
| Mg2+ | requirement | Homo sapiens | |
| Mg2+ | requirement | Saccharomyces cerevisiae | |
| Mg2+ | requirement | Triticum aestivum | |
| Mg2+ | 10 mM | Mus musculus | |
| Mg2+ | 10 mM | Homo sapiens | |
| Mn2+ | activation | Mus musculus | |
| Mn2+ | activation | Triticum aestivum | |
| additional information | no activation by Ca2+ | Mus musculus | |
| additional information | no activation by Ca2+ | Saccharomyces cerevisiae | |
| additional information | no activation by Ca2+ | Triticum aestivum | |
| additional information | no activation by Cu2+ and Zn2+ | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] | Mus musculus | presumably obligate part of transcription process | ADP + phospho-[DNA-directed RNA polymerase II subunit IIa] | - |
? | |
| ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] | Homo sapiens | presumably obligate part of transcription process | ADP + phospho-[DNA-directed RNA polymerase II subunit IIa] | - |
? | |
| ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] | Saccharomyces cerevisiae | presumably obligate part of transcription process | ADP + phospho-[DNA-directed RNA polymerase II subunit IIa] | - |
? | |
| ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] | Triticum aestivum | presumably obligate part of transcription process | ADP + phospho-[DNA-directed RNA polymerase II subunit IIa] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
| Saccharomyces cerevisiae | - |
baker's yeast | - |
| Triticum aestivum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| two kinases: cdc2/p58 and cdc2/p62 | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Mus musculus | - |
| cell culture | - |
Homo sapiens | - |
| cell culture | - |
Saccharomyces cerevisiae | - |
| Ehrlich ascites carcinoma cell | - |
Mus musculus | - |
| germ | - |
Triticum aestivum | - |
| HeLa cell | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.238 | - |
after second phenyl-superose chromatography | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + hepta-six peptide | - |
Mus musculus | ADP + phosphorylated hepta-six peptide | - |
? | |
| ATP + histone H1 | - |
Mus musculus | ADP + phosphohistone H1 | - |
? | |
| ATP + L-Arg-hepta peptide | - |
Mus musculus | ADP + phosphorylated L-Arg-hepta peptide | - |
? | |
| ATP + synthetic peptides | - |
Homo sapiens | ADP + ? | - |
? | |
| ATP + synthetic peptides | - |
Triticum aestivum | ADP + ? | - |
? | |
| ATP + synthetic peptides | hepta-six or Arg-hepta | Mus musculus | ADP + ? | - |
? | |
| ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] | no substrates are dTTP and AMP-PNP | Mus musculus | ADP + phospho-[DNA-directed RNA polymerase II subunit IIa] | - |
? | |
| ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] | phosphorylates predominantly Ser-residues | Homo sapiens | ADP + phospho-[DNA-directed RNA polymerase II subunit IIa] | - |
? | |
| ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] | phosphorylates predominantly Ser-residues | Saccharomyces cerevisiae | ADP + phospho-[DNA-directed RNA polymerase II subunit IIa] | - |
? | |
| ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] | phosphorylates predominantly Ser-residues | Triticum aestivum | ADP + phospho-[DNA-directed RNA polymerase II subunit IIa] | - |
? | |
| ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] | no substrates are CTP and UTP | Mus musculus | ADP + phospho-[DNA-directed RNA polymerase II subunit IIa] | - |
? | |
| ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] | no substrates are CTP and UTP | Triticum aestivum | ADP + phospho-[DNA-directed RNA polymerase II subunit IIa] | - |
? | |
| ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] | presumably obligate part of transcription process | Mus musculus | ADP + phospho-[DNA-directed RNA polymerase II subunit IIa] | - |
? | |
| ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] | presumably obligate part of transcription process | Homo sapiens | ADP + phospho-[DNA-directed RNA polymerase II subunit IIa] | - |
? | |
| ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] | presumably obligate part of transcription process | Saccharomyces cerevisiae | ADP + phospho-[DNA-directed RNA polymerase II subunit IIa] | - |
? | |
| ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] | presumably obligate part of transcription process | Triticum aestivum | ADP + phospho-[DNA-directed RNA polymerase II subunit IIa] | - |
? | |
| GTP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] | - |
Mus musculus | GDP + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | - |
Saccharomyces cerevisiae |
| More | enzymes from mouse consist of 2 components each: cdc2/p58: a cdc2 kinase (p34) and a p58 subunit, cdc2/p62: a cdc2 kinase and a p62 subunit (cyclin B), the latter is also called M phase-specific histone H1 kinase | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.2 | - |
Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | - |
Saccharomyces cerevisiae | |
| additional information | - |
Triticum aestivum | |
| additional information | no activation by cyclic nucleotides, phospholipids | Mus musculus |
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