Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | interaction of RV-cyclin with protein phosphatase 2A, overview | Homo sapiens | |
Protein phosphatase 2A | PP2A, enhances CDK8 kinase activity in vitro for the RNA polymerase II carboxy-terminal domain but not for histone H3. PP2A enhancement of CDK8 is independent of RV-cyclin expression and likely plays a role in the normal regulation of CDK8 | Homo sapiens | |
RV-cyclin | retroviral cyclin from Walleye dermal sarcoma virus, enhances CDK8 kinase activity in vitro for RNA polymerase II carboxy-terminal domain and histone H3. It is specific for cdk8, no activation of Cdk7 or Cdk9. The majority of the RV-cyclin structure is comprised of a cyclin box fold, which is responsible for binding to cyclin-dependent kinase-8. Two-hybrid interaction analysis in Saccharomyces cerevisiae strain Y190 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
transient expression of FLAG-CDK8 or kinase-deficient FLAG-CDK8 in HeLa cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | CDK8 is activated by autophosphorylation. protein phosphatase 2A regulates CDK8 autophosphorylation | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
colonic cancer cell | - |
Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cyclin H protein | CDK8 | Homo sapiens | ADP + cyclin H phosphoprotein | - |
? | |
ATP + E2F1 protein | CDK8 | Homo sapiens | ADP + E2F1 phosphoprotein | - |
? | |
ATP + histone H3 protein | CDK8 | Homo sapiens | ADP + histone H3 phosphoprotein | - |
? | |
ATP + Med13 protein | CDK8 | Homo sapiens | ADP + Med13 phosphoprotein | - |
? | |
ATP + RNA polymerase II carboxy-terminal domain | CDK8 | Homo sapiens | ADP + phosphorylated RNA polymerase II carboxy-terminal domain | - |
? | |
ATP + SMAD protein | CDK8 | Homo sapiens | ADP + SMAD phosphoprotein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CDK8 | - |
Homo sapiens |
cyclin-dependent kinase-8 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | PP2A enhancement of CDK8 is independent of RV-cyclin expression and likely plays a role in the normal regulation of CDK8 | Homo sapiens |