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Literature summary for 2.7.11.22 extracted from
- Activation of latent cyclin-dependent kinase 5 (Cdk5)-p35 complexes by membrane dissociation (2005), J. Neurochem., 94, 1535-1545.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| CHAPS | the detergent increases Cdk5-p35 activity with histone H1 and the autophosphorylation at p35 | Rattus norvegicus |  |
| NP-40 | the detergent increases Cdk5-p35 activity with histone H1 and the autophosphorylation at p35 | Rattus norvegicus | |
| p35 | regulatory subunit of Cdk5 | Rattus norvegicus | |
| Triton X-100 | the detergent increases Cdk5-p35 activity with histone H1 and the autophosphorylation at p35 | Rattus norvegicus | |
| Tween 20 | the detergent increases Cdk5-p35 activity with histone H1 and the autophosphorylation at p35 | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| membranes | of brain and liver, Cdk5-p35 is negatively regulated by interaction with membranes, mechanism | Rattus norvegicus | |
| roscovitine | - |
Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | Cdk5-p35 is negatively regulated by interaction with membranes in brain and liver, mechanism | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
Wistar rats | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | Cdk5 autophosphorylates at its cofactor p35 which activates the complex | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial purification of Cdk5-p35 from brain homogenates, solubilization by NP-40 0.5% in presence of 0.6 M NaCl | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + histone H1 | - |
Rattus norvegicus | ADP + phosphorylated histone H1 | - |
? | |
| ATP + NF-H peptide | i.e. RREAKSPAKAKSPAKE | Rattus norvegicus | ADP + phosphorylated NF-H peptide | - |
? | |
| additional information | Cdk5-p35 is negatively regulated by interaction with membranes in brain and liver, mechanism | Rattus norvegicus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cdk5-p35 | - |
Rattus norvegicus |
| cyclin-dependent kinase 5-p35 | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Rattus norvegicus | |
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