Literature summary for 2.7.11.21 extracted from

Chauhan, S.; Samanta, S.; Sharma, N.; Thakur, J.K.; Dev, K.; Sourirajan, A.
Saccharomyces cerevisiae polo-like kinase, Cdc5 exhibits ATP-dependent Mg2+-enhanced kinase activity in vitro (2019), Heliyon, 5, e03050 .

Search for more literature for 2.7.11.21

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	5 mM Ca2+ inhibits the enzyme activity	Saccharomyces cerevisiae
EDTA	-	Saccharomyces cerevisiae
Mn2+	10 mM Ca2+ inhibits the enzyme activity, 5 mM is used in assay conditions	Saccharomyces cerevisiae
additional information	not inhibited by dithiothreitol	Saccharomyces cerevisiae
phenylmethylsulfonyl fluoride	-	Saccharomyces cerevisiae
SDS	-	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00135	-	casein	at pH 7.5 and 30°C	Saccharomyces cerevisiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	20 mM Mg2+ enhances the enzyme activity, 10 mM is used in assay conditions	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + bovine serum albumin	very weak phosphorylation	Saccharomyces cerevisiae	ADP + phosphorylated bovine serum albumin	-	?
ATP + casein	best in vitro substrate	Saccharomyces cerevisiae	ADP + phosphorylated casein	-	?
ATP + myelin basic protein	very weak phosphorylation	Saccharomyces cerevisiae	ADP + phosphorylated myelin basic protein	-	?

Subunits

Subunits	Comment	Organism
?	x * 107000, GST-tagged enzyme, SDS-PAGE	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
Cdc5	-	Saccharomyces cerevisiae
PLK	-	Saccharomyces cerevisiae
polo-like kinase	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Saccharomyces cerevisiae

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)