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Literature summary for 2.7.11.21 extracted from

  • Yu, Z.; Liu, Y.; Li, Z.
    Structure-function relationship of the Polo-like kinase in Trypanosoma brucei (2012), J. Cell Sci., 125, 1519-1530.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.21

Cloned(Commentary)

Cloned (Comment) Organism
the Trypanosoma brucei EYFP-tagged enzyme is ectopically expressed in HeLa cells. It is predominantly nuclear in interphase cells, whereas in mitotic cells it is localized throughout the cytoplasm. It is unable to be targeted to spindle poles, the central spindle or the midbody in HeLa cells Trypanosoma brucei

Protein Variants

Protein Variants Comment Organism
F561A mutation does not affect kinase activity, the mutant enzyme is correctly localized to the anterior tip of the new flagellum attachment zone Trypanosoma brucei
H710A mutation does not affect kinase activity, the mutant enzyme is correctly localized to the anterior tip of the new flagellum attachment zone Trypanosoma brucei
K70R no kinase activity Trypanosoma brucei
K712A mutation does not affect kinase activity, the mutant enzyme is correctly localized to the anterior tip of the new flagellum attachment zone Trypanosoma brucei
additional information deletion of the C-terminal polo-box domain abolishes localization of polo-like kinase Trypanosoma brucei
T198A mutation disrupts the ability to phosphorylate the in vitro substrate TbCentrin2 Trypanosoma brucei
T198D kinase activity of the mutant enzyme is several-fold higher than that of wild-type enzyme Trypanosoma brucei
T201A mutation disrupts the ability to phosphorylate the in vitro substrate TbCentrin2 Trypanosoma brucei
T201D kinase activity of the mutant enzyme is several-fold higher than that of wild-type enzyme Trypanosoma brucei
W557F mutation does not affect kinase activity, the mutant enzyme is correctly localized to the anterior tip of the new flagellum attachment zone Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
flagellum the enzyme is enriched at the anterior tip of the flagellum attachment zone Trypanosoma brucei
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-

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + protein p110
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 Trypanosoma brucei ADP + ?
-
 ?
ATP + TbCentrin2
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 Trypanosoma brucei ADP + ?
-
 ?

Synonyms

Synonyms Comment Organism
TbPLK
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 Trypanosoma brucei