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Literature summary for 2.7.11.21 extracted from
- Functional dynamics of Polo-like kinase 1 at the centrosome (2009), Mol. Cell. Biol., 29, 3134-3150.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Plk1 cDNAs are subcloned into pEGFP-C1 | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| EGFP-Plk1D176N | construct used in FRAP experiments, expressed in U2OS cells, kinase-dead mutant | Homo sapiens |
| EGFP-Plk1PBD | construct used in FRAP experiments, expressed in U2OS cells, only C-terminal PBD domain | Homo sapiens |
| EGFP-Plk1T210A | construct used in FRAP experiments, expressed in U2OS cells, nonphosphorylatable T-loop form of Plk1 | Homo sapiens |
| EGFP-Plk1T210D | construct used in FRAP experiments, expressed in U2OS cells, constitutively active mutant | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| centrosome | - |
Homo sapiens | 5813 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + a protein | Homo sapiens | - |
ADP + a phosphoprotein | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P53350 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + a protein | - |
Homo sapiens | ADP + a phosphoprotein | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Plk1 | - |
Homo sapiens |
| Polo-like kinase 1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
kinase assay | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
kinase assay | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | Plk1 function as a key regulator of mitotic events by phosphorylating substrate proteins of centrosomes, kinetochores, the mitotic spindle, and the midbody | Homo sapiens |
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Release 2023.1 (January 2023)
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