Application | Comment | Organism |
---|---|---|
medicine | the data demonstrate the function of Plk1 as a regulator of interferon induction and provide the basis for the development of inhibitors preventing the PLK1/MAVS association to sustain innate immunity | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
a full-length Myc-PLK1 plasmid is used, full-length PLK1, cloned into pDON207, is inserted into a pCINeo vector, pFLAG-PLK1-PBD and pEGFP-PLK1-PBD constructs are generated | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
PLK1-PBD H538A/K540M | PLK1 polo box domain double mutant, constructed for the determination of the specificity of the association of PLK1 with MAVS | Homo sapiens |
PLK1-PBD W414F/H538A/K540M | PLK1 polo box domain triple mutant, constructed for the determination of the specificity of the association of PLK1 with MAVS | Homo sapiens |
T210D | mutation in sequence of full-lenght construct | Homo sapiens |
T210V | mutation in sequence of full-lenght construct | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
determined by SDS-PAGE and Western Blot analysis | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a protein | Homo sapiens | - |
ADP + a phosphoprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P53350 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293T cell | - |
Homo sapiens | - |
MCF-7 cell | - |
Homo sapiens | - |
nasopharyngeal carcinoma cell | screening of a lambdagt 10 library, full-length construct | Homo sapiens | - |
spleen | cDNA library screen, polo-box domain | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a protein | - |
Homo sapiens | ADP + a phosphoprotein | - |
? | |
ATP + protein MAVS | phosphorylation of the mitochondria-bound adapter protein MAVS, MAVS, mitochondrial antiviral signaling | Homo sapiens | ADP + phosphorylated protein MAVS | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Plk1 | - |
Homo sapiens |
Polo-like kinase 1 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | Plk1 is a regulator of interferon induction | Homo sapiens |