Application | Comment | Organism |
---|---|---|
additional information | temporal control of HsCdc14A phosphatase activity by PLK1 is essential for chromosome segregation in mitosis | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysS as a GST fusion protein, 293T cells co-transfected with FLAG-PLK1 and GFPHsCdc14A, GST-tagged PLK1 kinase expressed in insect, HeLa cells transiently transfected to express GFP-HsCdc14A and FLAG-PLK1 | Homo sapiens |
into the pGEX-6P vector for expression in Escherichia coli BL21DE3 cells, furthermore an insect expression construct is used, and in fusion with a FLAG-tag for transfection of HEK-293T cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
PLK1-C | construct comprising amino acids 305-603 | Homo sapiens |
PLK1-N | construct comprising amino acids 1-300 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
siRNA | reduces serine phosphorylation of HsCdc14A but not HsCdc14 protein level | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
centrosome | - |
Homo sapiens | 5813 | - |
centrosome | co-distributes with HsCdc14A to the centrosomes from prophase to metaphase | Homo sapiens | 5813 | - |
chromosome | - |
Homo sapiens | 5694 | - |
additional information | at telophase, PLK1 localization is only at the mid-body where HsCdc14A is also apparent | Homo sapiens | - |
- |
spindle | - |
Homo sapiens | 5819 | - |
spindle | by anaphase, PLK1 relocates from the centrosome to the central spindle while HsCdc14A is still at centrosome | Homo sapiens | 5819 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + protein | Homo sapiens | - |
ADP + phosphoprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | P53350 | - |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration | Homo sapiens |
using glutathione-agarose | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293T cell | - |
Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Cdc14A | - |
Homo sapiens | ADP + phosphorylated Cdc14A | - |
? | |
ATP + HsCdc14A | HsCdc14A binds to PLK1 via its C-terminus, phosphorylation partially releases the auto-inhibition of HsCdc14A, PLK1-mediated phospho-regulation promotes HsCdc14A phosphatase activity | Homo sapiens | ADP + phosphorylated HsCdc14A | - |
? | |
ATP + protein | - |
Homo sapiens | ADP + phosphoprotein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Plk1 | - |
Homo sapiens |
Polo-like kinase 1 | - |
Homo sapiens |