Application | Comment | Organism |
---|---|---|
medicine | correlation between Plk1 overexpression in tumors and poor patient prognosis, Plk1 acts at multiple discrete steps throughout late G2 phase, mitosis, and cytokinesis, triggers activation of the Ect2-RhoA network | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | both copies of the PLK1 locus deleted in telomerase-immortalized human retinal pigment epithelial cells, more than 90% knockdown of Plk1 does not cause any cell cycle impairment, PLK1-null cells arrest in mitosis with immature centrosomes and monopolar spindles. Plk1 function and cell viability can be reconstituted by expressing either wildtype Plk1 or an analog-sensitive variant, the analog-sensitive variant is inhibited by 3-MB-PP1 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
BI-2536 | potential anti-cancer therapeutic agent, shows strong selectivity for Plk1 | Homo sapiens | |
BTO-1 | - |
Homo sapiens | |
additional information | inhibited by Plk1-specific antibodies | Homo sapiens | |
RNA | RNA interference abrogates centrosome maturation, spindle bipolarization, and silencing of the spindle assembly checkpoint | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
kinetochore | rapidly accumulates at kinetochores, appears to be partially released once all chromosomes are properly congressed at the metaphase plate | Homo sapiens | 776 | - |
microtubule | at anaphase onset, Plk1 redistributes onto equatorial microtubule bundles that define the spindle midzone or central spindle, and remain tightly associated with these microtubules during midbody formation and completion of cytokinesis | Homo sapiens | 5874 | - |
spindle | rapidly accumulates at spindle poles | Homo sapiens | 5819 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P53350 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
carcinoma cell | increased requirement for Plk1 in comparison to normal cells | Homo sapiens | - |
mitotic cell | Plk1 abundance and kinase activity are strongly upregulated at the G2/M transition, reach a peak during mitosis, and are downregulated by the subsequent G1 phase, albeit with slower kinetics than Cdk1/cyclin B activity, which falls acutely at anaphase onset | Homo sapiens | - |
retinal pigment epithelium | - |
Homo sapiens | - |
somatic cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Ect2 | - |
Homo sapiens | ADP + phosphorylated Ect2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Plk1 | - |
Homo sapiens |
Polo-like kinase 1 | - |
Homo sapiens |