Literature summary for 2.7.11.21 extracted from

Garcia-Alvarez, B.; Ibanez, S.; Montoya, G.
Crystallization and preliminary X-ray diffraction studies on the human Plk1 Polo-box domain in complex with an unphosphorylated and a phosphorylated target peptide from Cdc25C (2006), Acta Crystallogr. Sect. F, 62, 372-375.

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Cloned(Commentary)

Cloned (Comment)	Organism
Plk1 polo-box domain cloned into vector pGEX-6P-2, overexpressed as a GST fusion protein in Escherichia coli BL21(DE3) plys strain	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
Plk1 polo-box domain in complex with unphosphorylated and phosphorylated Cdc25C, to 2.1 and 2.85 A resolution, respectively, by the sitting-drop method, crystals of the polo-box domain in complex with the phosphorylated peptide belong to the orthorhombic space group P212121, with unit-cell parameters a = 38.23, b = 67.35, c = 88.25 A, alpha = gamma = beta = 90°, and contain one molecule per asymmetric unit. Crystals of the polo-box domain in complex with the unphosphorylated peptide belong to the monoclinic space group P21, with unit-cell parameters a = 40.18, b = 49.17, c = 56.23 A, alpha = gamma = 90°, beta = 109.48°, and contain one molecule per asymmetric unit	Homo sapiens

Crystallization (Comment)

Organism

Plk1 polo-box domain in complex with unphosphorylated and phosphorylated Cdc25C, to 2.1 and 2.85 A resolution, respectively, by the sitting-drop method, crystals of the polo-box domain in complex with the phosphorylated peptide belong to the orthorhombic space group P212121, with unit-cell parameters a = 38.23, b = 67.35, c = 88.25 A, alpha = gamma = beta = 90°, and contain one molecule per asymmetric unit. Crystals of the polo-box domain in complex with the unphosphorylated peptide belong to the monoclinic space group P21, with unit-cell parameters a = 40.18, b = 49.17, c = 56.23 A, alpha = gamma = 90°, beta = 109.48°, and contain one molecule per asymmetric unit

Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
26000	-	recombinant human Plk1 polo-box domain	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P53350	-	-

Purification (Commentary)

Purification (Comment)	Organism
by gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + Cdc25C	-	Homo sapiens	ADP + phosphorylated Cdc25C	-	?

Synonyms

Synonyms	Comment	Organism
Plk1	-	Homo sapiens
Polo-like kinase 1	-	Homo sapiens