Cloned (Comment) | Organism |
---|---|
expression in U2OS cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Plk1 depletion by siRNA transfection combined with Nek2 overexpression in U2OS cells | Homo sapiens |
T210D | site-directed mutagenesis, constitutively active mutant, overrides the irradiation-induced DNA damage and inhibition of centrosome separation in contrast to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | in vivo inhibition of Plk1 by DNA damage is ATM- or ATR-dependent | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | - |
Homo sapiens | |
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme is involved in regulation of Nek2-induced centrosome separation after DNA damage | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
U2-OS cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + casein | - |
Homo sapiens | ADP + phosphorylated casein | - |
? | |
additional information | the enzyme is involved in regulation of Nek2-induced centrosome separation after DNA damage | Homo sapiens | ? | - |
? | |
additional information | the protein phosphatase 1 is no substrate of Plk1 | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Plk1 | - |
Homo sapiens |
Polo-like kinase 1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |