Cloned (Comment) | Organism |
---|---|
stable expression of Plk1 and transfection with hairpin shRNA in HeLa cells, expression of GST-tagged Plk1 in Spodoptera frugiperda Sf9 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K82M | site-directed mutagenesis, kinase-defective Plk1 mutant | Homo sapiens |
additional information | down-regulation of Plk1 by expression of hairpin shRNA results in increased Pin1 ubiquitination and to down-regulation of pin 1 levels during mitosis | Homo sapiens |
T210D | site-directed mutagenesis, hyperactive Plk1 mutant, shows reduced activity with truncated Pin1 compared to the wild-tpe Plk1 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Pin1 | Homo sapiens | Plk1-mediated phosphorylation at Ser65 stabilizes Pin1 by inhibiting its ubiquitination in cells | ADP + phosphorylated Pin1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P53350 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged Plk1 from Spodoptera frugiperda Sf9 cells | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293T cell | - |
Homo sapiens | - |
HeLa-S3 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Pin1 | Plk1-mediated phosphorylation at Ser65 stabilizes Pin1 by inhibiting its ubiquitination in cells | Homo sapiens | ADP + phosphorylated Pin1 | - |
? | |
ATP + Pin1 | recombinant isomerase Pin1 expressed in Escherichia coli, phosphorylation at Ser65, wild-type and truncated mutant Pin1 comprising residues 1-70 | Homo sapiens | ADP + phosphorylated Pin1 | - |
? | |
ATP + Plk1 polo box 1-derived peptide | synthetic substrate | Homo sapiens | ADP + phosphorylated Plk1 polo box 1-derived peptide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Plk1 | - |
Homo sapiens |
Polo-like kinase 1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |