Cloned (Comment) | Organism |
---|---|
expression of His6-tagged wild-type and mutant Plk3 in HeLa cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K52R | site-directed mutagenesis, kinase-defective mutant | Homo sapiens |
additional information | construction of an kinase-active deletion mutant lacking 32 amino acids of te N-terminus | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | localization of Plk3 with Golgi fragments during mitosis, overview | Homo sapiens | 5794 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens | |
Mn2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, about, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + giantin | Homo sapiens | - |
ADP + phosphorylated giantin | - |
? | |
additional information | Homo sapiens | Plk3 is involved in regulating Golgi fragmentation during the cell cycle | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type and mutant Plk3 from HeLa cells | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-549 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-casein | - |
Homo sapiens | ADP + phosphorylated alpha-casein | - |
? | |
ATP + giantin | - |
Homo sapiens | ADP + phosphorylated giantin | - |
? | |
additional information | Plk3 is involved in regulating Golgi fragmentation during the cell cycle | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, about, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Plk3 | - |
Homo sapiens |
polo-like kinase 3 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |