Cloned (Comment) | Organism |
---|---|
expression of GST-tagged Plx1 in S2 cells, co-expression with GFP-tagged MEI-S332 | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
additional information | male homozygous polo mutant flies die in the third instar larval stage, heterozygous mutants show affected centromer dissociation of MEI-S332, but not association resulting in chromosome segragation defects | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MEI-S332 protein | Xenopus laevis | phosphorylation/polo box binding is required for chromosomal dissociation of MEI-S332 | ADP + phosphorylated MEI-S332 protein | - |
? | |
ATP + MEI-S332 protein | Drosophila melanogaster | substrate is a protein essential in meiosis for maintaining cohesion at centromers until sister chromatids separate at the metaphase II/anaphase II transition, phosphorylation by polo kinase removes the protein from centromeres and antagonizes the MEI-S332 function, MEI-S332 phosphorylation by polo kinase is essential for viability of the cells | ADP + phosphorylated MEI-S332 protein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
gene polo | - |
Xenopus laevis | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
egg | - |
Xenopus laevis | - |
SCHNEIDER-2 cell | embryonic cell line | Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MEI-S332 protein | phosphorylation/polo box binding is required for chromosomal dissociation of MEI-S332 | Xenopus laevis | ADP + phosphorylated MEI-S332 protein | - |
? | |
ATP + MEI-S332 protein | substrate is a protein essential in meiosis for maintaining cohesion at centromers until sister chromatids separate at the metaphase II/anaphase II transition, phosphorylation by polo kinase removes the protein from centromeres and antagonizes the MEI-S332 function, MEI-S332 phosphorylation by polo kinase is essential for viability of the cells | Drosophila melanogaster | ADP + phosphorylated MEI-S332 protein | - |
? | |
ATP + MEI-S332 protein | enzyme requires residues T331 and possibly in combination with S234 for activity, no activity with MEI-S332 protein mutants T331A and S234A/T331A | Xenopus laevis | ADP + phosphorylated MEI-S332 protein | - |
? | |
ATP + MEI-S332 protein | enzyme requires residues T331 and possibly in combination with S234 for activity, no activity with MEI-S332 protein mutants T331A and S234A/T331A in S2 cells | Drosophila melanogaster | ADP + phosphorylated MEI-S332 protein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Plx1 | - |
Xenopus laevis |
Polo kinase | - |
Drosophila melanogaster |
Polo-like kinase 1 | - |
Xenopus laevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Drosophila melanogaster | |
ATP | - |
Xenopus laevis |