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Literature summary for 2.7.11.20 extracted from

  • Tavares, C.D.; Giles, D.H.; Stancu, G.; Chitjian, C.A.; Ferguson, S.B.; Wellmann, R.M.; Kaoud, T.S.; Ghose, R.; Dalby, K.N.
    Signal integration at elongation factor 2 kinase the roles of calcium, calmodulin, and Ser-500 phosphorylation (2017), J. Biol. Chem., 292, 2032-2045 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.20

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin absolutely necessary for efficient enzyme activity Homo sapiens

Protein Variants

Protein Variants Comment Organism
D284A catalytically inactive Homo sapiens
S500D the mutation enhances the rate of activation (Thr-348 autophosphorylation) by 6fold and lowers the EC50 for Ca2+/calmpdulin binding to activated enzyme (Thr-348 phosphorylated) by 20fold as compared to the wild type Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+ enhances the affinity of calmodulin toward the enzyme, 0.15 mM Ca2+ is used in assay conditions Homo sapiens
Mg2+ 10 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [elongation factor 2] Homo sapiens
-
 ADP + [elongation factor 2] phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O00418
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the enzyme autophosphorylates at Thr-348. Phosphorylation of Ser-500 integrates with Ca2+ and calmodulin to regulate the enzyme activity. Phosphorylation of Thr-348 and Ser-500 regulates the Ca2+/calmodulin-dependent activity of the enzyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293T cell
-
 Homo sapiens
-
MCF-10A cell
-
 Homo sapiens
-
MDA-MB-231 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [elongation factor 2]
-
 Homo sapiens ADP + [elongation factor 2] phosphate
-
 ?

Synonyms

Synonyms Comment Organism
EEF-2K
-
 Homo sapiens
eukaryotic elongation factor 2 kinase
-
 Homo sapiens