Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | required | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Rosetta 2 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
T482A | catalytically inactive mutant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Homo sapiens | |
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [elongation factor 2] | Homo sapiens | - |
ADP + [elongation factor 2] phosphate | - |
? | |
additional information | Homo sapiens | mammalian enzyme elongation factor-2 kinase (eEF2K) a requirement for threonine residues as the target of phosphorylation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O00418 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the enzyme performs autophosphorylation at Thr57 | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 Rosetta 2 by nickel affinity chromatography, ultrafiltration, cleavage of the tag with Sumo protease, and anion exchange chromatography, followed by gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cortactin | i.e. SRC8, an Src substrate, phosphorylation peptide sequence is EYQGK-T-EKHAS | Homo sapiens | ADP + phosphorylated cortactin | - |
? | |
ATP + heat shock protein 60 | i.e. HSPD1, phosphorylation peptide sequence is TKDGV-T-VAKSI | Homo sapiens | ADP + phosphorylated heat shock protein 60 | - |
? | |
ATP + hematological and neurological expressed 1-like protein | i.e. HN1L, phosphorylation peptide sequence is FGSPV-T-ATSRL | Homo sapiens | ADP + phosphorylated hematological and neurological expressed 1-like protein | - |
? | |
ATP + heterogeneous nuclear ribonucleoprotein A1 | i.e. HNRNPA1, phosphorylation peptide sequence is VSRED-S-QRPGA | Homo sapiens | ADP + phosphorylated heterogeneous nuclear ribonucleoprotein A1 | - |
? | |
ATP + immunoglobulin-binding protein 1 | i.e. IGBP1, phosphorylation peptide sequence is EDDEQ-T-LHRAR | Homo sapiens | ADP + phosphorylated immunoglobulin-binding protein 1 | - |
? | |
ATP + microtubule-associated protein 4 | i.e. MAP4, phosphorylation peptide sequence is TEAAA-T-TRKPE | Homo sapiens | ADP + phosphorylated microtubule-associated protein 4 | - |
? | |
ATP + N-myc downstream-regulated gene 1 protein | i.e. NDRG1, phosphorylation peptide sequence is TSLDG-T-RSRSH | Homo sapiens | ADP + phosphorylated N-myc downstream-regulated gene 1 protein | - |
? | |
ATP + PEST proteolytic signal-containing nuclear protein | i.e. PCNP, phosphorylation peptide sequence is AIGSQ-T-TKKAS | Homo sapiens | ADP + phosphorylated PEST proteolytic signal-containing nuclear protein | - |
? | |
ATP + prothymosin alpha | i.e. PTMA, phosphorylation peptide sequence is TSSEI-T-TKDLK | Homo sapiens | ADP + phosphorylated prothymosin alpha | - |
? | |
ATP + SAP domain-containing ribonucleoprotein | i.e. SARNP, phosphorylation peptide sequence is GTTED-T-EAKKR | Homo sapiens | ADP + phosphorylated SAP domain-containing ribonucleoprotein | - |
? | |
ATP + scaffold attachment factor B2 | i.e. SAFB2, phosphorylation peptide sequence is VISVK-T-TSRSK | Homo sapiens | ADP + phosphorylated scaffold attachment factor B2 | - |
? | |
ATP + U4/U6 small nuclear ribonucleoprotein Prp31 | i.e. PRP31, phosphorylation peptide sequence is ERLGL-T-EIRKQ | Homo sapiens | ADP + phosphorylated U4/U6 small nuclear ribonucleoprotein Prp31 | - |
? | |
ATP + [elongation factor 2] | - |
Homo sapiens | ADP + [elongation factor 2] phosphate | - |
? | |
additional information | mammalian enzyme elongation factor-2 kinase (eEF2K) a requirement for threonine residues as the target of phosphorylation | Homo sapiens | ? | - |
? | |
additional information | the enzyme performs autophosphorylation at Thr57. Additional enzyme protein substrates show a strong sequence motif consisting of acidic residues in the -2 position and basic residues in the +3 position and almost exclusively threonine as the phosphorylated residue, peptide mapping and mass spectrometry analysis, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
eEF2 kinase | - |
Homo sapiens |
eEF2K | - |
Homo sapiens |
elongation factor-2 kinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
ATPgammaS | i.e. ATP-gamma-thiophosphate | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | eEF2K is a calcium/calmodulin dependent kinase that belongs to the alpha kinase family, which has negligible sequence identity to the conventional protein kinase family | Homo sapiens |
additional information | homology modelling of eEF2K using the structure of myosin heavy chain kinase, EC 2.7.11.7, PDB ID 3LMH. A glutamate might act as gatekeeper and makes likely hydrogen bonds to the adenine in the active site and is essential for activity in the alpha kinase family | Homo sapiens |
physiological function | the phosphorylation of eEF2 through eEF2 kinase blocks translation elongation, which is thought to be critical to regulating cellular energy usage. eEF2K has a role in regulating cellular energy usage that involves multiple pathways and regulatory feedback | Homo sapiens |