Literature summary for 2.7.11.20 extracted from

Pyr Dit Ruys, S.; Wang, X.; Smith, E.M.; Herinckx, G.; Hussain, N.; Rider, M.H.; Vertommen, D.; Proud, C.G.
Identification of autophosphorylation sites in eukaryotic elongation factor-2 kinase (2012), Biochem. J., 442, 681-692.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression as a GST (glutathione transferase)-fusion protein in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
S366A	activity with RKKFGESEKTKTKEFL is decreased approximately 90% compared to wild-type activity after preincubation for 60 min with MgATP2-	Homo sapiens
S78A	activity with RKKFGESEKTKTKEFL is decreased approximately 50% compared to wild-type activity after preincubation for 60 min with MgATP2-	Homo sapiens
T348A	activity with RKKFGESEKTKTKEFL is decreased approximately 90% compared to wild-type activity after preincubation for 60 min with MgATP2-. The mutant enzyme phosphorylates purified elongation factor 2 poorly compared with wild-type	Homo sapiens
T348D	the mutant enzyme phosphorylates purified elongation factor 2 poorly compared with wild-type	Homo sapiens
T348E	the mutant enzyme phosphorylates purified elongation factor 2 poorly compared with wild-type	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	Ca2+/calmodulin-dependent enzyme	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	phosphorylation sites: Ser61, Ser66, Ser78, Ser366, Ser445, Ser474, Ser491, Thr348, Thr353. The autophosphorylation sites Thr348, Thr353, Ser366 and Ser445 are are highly conserved among vertebrate elongation factor 2 kinases. None of the sites lies in the catalytic domain. Ser366 is phosphorylated not only autocatalytically but also in trans by other kinases. Thr348 appears to be constitutively autophosphorylated in vitro. Thr348, and presumably its autophosphorylation, are critical for the ability of the enzyme to phosphorylate substrates in trans	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + RKKFGESEKTKTKEFL	-	Homo sapiens	ADP + ?	-	?
ATP + [elongation factor 2]	Thr348, and presumably its autophosphorylation, are critical for the ability of the enzyme to phosphorylate substrates in trans	Homo sapiens	ADP + [elongation factor 2] phosphate	-	?

Synonyms

Synonyms	Comment	Organism
eEF2K	-	Homo sapiens

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Release 2023.1 (January 2023)