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Literature summary for 2.7.11.2 extracted from

  • Reed, L.J.; Damuni, Z.; Merryfield, M.L.
    Regulation of mammalian pyruvate and branched-chain alpha-keto acid dehydrogenase complexes by phosphorylation-dephosphorylation (1985), Curr. Top. Cell. Regul., 27, 41-49.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-oxoisopentanoate
-
Bos taurus
acetyl-CoA
-
Bos taurus
NADH activation, in the presence of NH4+ Bos taurus
NADH activation, in the presence of K+ Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
ADP inhibition only in the presence of monovalent cations Bos taurus
Disulfides thiols reverse Bos taurus
DTNB
-
Bos taurus
NEM
-
Bos taurus
pyruvate
-
Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
Mg2+
-
Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion inner membrane matrix compartment Bos taurus 5739
-
mitochondrion tightly bound to dihydrolipoamide acetyltransferase of pyruvate dehydrogenase complex Bos taurus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Bos taurus
Mn2+ requirement Bos taurus
Mn2+ can replace Mg2+ to some extent Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
1 * 48000, alpha + 1 * 45000, beta, SDS-PAGE Bos taurus
48000
-
1 * 48000, alpha + 1 * 45000, beta, SDS-PAGE Bos taurus
100000
-
-
Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [pyruvate dehydrogenase (lipoamide)] Bos taurus catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
kidney, from highly purifed pyruvate dehydrogenase-complex Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
Bos taurus
-
kidney
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [pyruvate dehydrogenase (lipoamide)] highly specific for the substrate Bos taurus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir
ATP + [pyruvate dehydrogenase (lipoamide)] phosphorylation sites are 3 Ser-residues in the alpha-subunit, i.e. E1, MW 41000, of pyruvate dehydrogenase Bos taurus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir
ATP + [pyruvate dehydrogenase (lipoamide)] catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 Bos taurus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir
additional information no activity with glycogen synthase a and rabbit skeletal muscle phosphorylase b Bos taurus ?
-
?

Subunits

Subunits Comment Organism
dimer 1 * 48000, alpha + 1 * 45000, beta, SDS-PAGE Bos taurus
More kinase activity resides in alpha-subunit Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.533
-
ATP
-
Bos taurus

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Bos taurus