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Literature summary for 2.7.11.2 extracted from

  • Hirani, T.; Tovar-Mendez, A.; Miernyk, J.; Randall, D.
    Asp295 stabilizes the active-site loop structure of pyruvate dehydrogenase, facilitating phosphorylation of Ser292 by pyruvate dehydrogenase-kinase (2011), Enzyme Res., 2011, 939068.
    View publication on PubMedView publication on EuropePMC

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial matrix
-
Arabidopsis thaliana 5759
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [pyruvate dehydrogenase (acetyl-transferring)] Arabidopsis thaliana phosphorylation at Ser292 by PDK1 ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate
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r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [pyruvate dehydrogenase (acetyl-transferring)] phosphorylation at Ser292 by PDK1 Arabidopsis thaliana ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate
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r
ATP + [pyruvate dehydrogenase (acetyl-transferring)] reversible phosphorylation of alpha2beta2-heterotetrameric pyruvate dehydrogenase complex, i.e. E1, phosphorylation at Ser292 within the active-site loop structure of E1alpha by PDK1. Substrate mutation of Asp295 to Ala, Asn, or Leu greatly reduces phosphorylation of Ser292, while mutation of Gly297 has relatively little effect. AtPDC E1alpha S298A mutant is phosphorylated by AtPDK. Activity of the enzyme with different substrate mutants, overview Arabidopsis thaliana ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate
-
r
additional information pyruvate dehydrogenase Asp295 plays an important role in stabilizing the active-site loop structure of the pyruvate dehydrogenase, facilitating transfer of the gamma-phosphate from ATP to the Ser residue at regulatory site one of E1alpha, substrate structure, overview Arabidopsis thaliana ?
-
?

Synonyms

Synonyms Comment Organism
E1-kinase
-
Arabidopsis thaliana
PDK
-
Arabidopsis thaliana
pyruvate dehydrogenase-kinase
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Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
Arabidopsis thaliana