Literature summary for 2.7.11.2 extracted from

Krus, U.; Kotova, O.; Spegel, P.; Hallgard, E.; Sharoyko, V.V.; Vedin, A.; Moritz, T.; Sugden, M.C.; Koeck, T.; Mulder, H.
Pyruvate dehydrogenase kinase 1 controls mitochondrial metabolism and insulin secretion in INS-1 832/13 clonal beta-cells (2010), Biochem. J., 429, 205-213.

Search for more literature for 2.7.11.2

Protein Variants

Protein Variants	Comment	Organism
additional information	PDH kinase 1 knockout with siRNA, siRNA transfection into 832/13 clonal beta-cells, transfectant metabolite profiling compared to control cells, overview	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrial matrix	-	Rattus norvegicus	5759	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + [pyruvate dehydrogenase (acetyl-transferring)]	Rattus norvegicus	-	ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
INS-1 832/13 cell	-	Rattus norvegicus	-
pancreatic beta cell	-	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + [pyruvate dehydrogenase (acetyl-transferring)]	-	Rattus norvegicus	ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate	-	?

Synonyms

Synonyms	Comment	Organism
PDH kinase 1	-	Rattus norvegicus
pyruvate dehydrogenase kinase 1	-	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Rattus norvegicus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Rattus norvegicus

General Information

General Information	Comment	Organism
malfunction	knockdown of PDH kinase 1 does not affect basal insulin secretion but, remarkably, insulin secretion in response to 16.7 mM glucose increases more vigorously than in negative control cells. The metabolite profile of cells with PDH kinase 1 knockdown is significantly different from that of the control cells, expecially the tricarboxylic acid cycle intermediates malate, fumarate and 2-oxoglutarate, overview	Rattus norvegicus
metabolism	metabolic profiling and glucose-stimulated insulin secretion in pancreatic beta cells with and without knockdown of the enzyme, overview	Rattus norvegicus

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Release 2023.1 (January 2023)