Protein Variants | Comment | Organism |
---|---|---|
R250A | catalytic activity is markedly reduced, Kd value for ATP is 32fold increased, resistant to the inhibitory effect of dichloroacetate, a synthetic analogue of pyruvate | Rattus norvegicus |
T302A | catalytic activity is considerably reduced, Kd value for ATP is 24fold increased, resistant to the inhibitory effect of dichloroacetate, a synthetic analogue of pyruvate | Rattus norvegicus |
Y320F | catalytic activity is slightly reduced, Kd value for ATP is decreased to 68%, resistant to the inhibitory effect of dichloroacetate, a synthetic analogue of pyruvate | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | Q64536 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.045 | - |
PDHK2 mutant T302A | Rattus norvegicus |
0.17 | - |
PDHK2 mutant R250A | Rattus norvegicus |
0.46 | - |
PDHK2 mutant Y320F | Rattus norvegicus |
0.53 | - |
wild type PDHK2 | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate dehydrogenase | Kd for ATP is 2.5 micromol, for ADP 10.6 micromol and for dichloroacetate 226 micromol | Rattus norvegicus | ADP + phosphorylated pyruvate dehydrogenase | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PDHK2 | - |
Rattus norvegicus |
pyruvate dehydrogenase kinase 2 | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
physiological function | phosphorylation (activation) of pyruvate dehydrogenase | Rattus norvegicus |