Cloned (Comment) | Organism |
---|---|
PDK DNA sequence determination and analysis, expression of His6-tagged wild-type and mutant PDKs in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
E238A | site-directed mutagenesis, highly reduced activity compared to the wild-type PDK | Arabidopsis thaliana |
E238H | site-directed mutagenesis, highly reduced activity compared to the wild-type PDK | Arabidopsis thaliana |
E238Q | site-directed mutagenesis, highly reduced activity compared to the wild-type PDK | Arabidopsis thaliana |
H233A | site-directed mutagenesis, reduced activity compared to the wild-type PDK | Arabidopsis thaliana |
K241A | site-directed mutagenesis, highly reduced activity compared to the wild-type PDK | Arabidopsis thaliana |
L234A | site-directed mutagenesis, reduced activity compared to the wild-type PDK | Arabidopsis thaliana |
L234H | site-directed mutagenesis, reduced activity compared to the wild-type PDK | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Arabidopsis thaliana | |
0.05 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant PDK mutant L234H | Arabidopsis thaliana | |
0.05 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant wild-type PDK | Arabidopsis thaliana | |
0.075 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant PDK mutant L234A | Arabidopsis thaliana | |
0.2 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant PDK mutant H233A | Arabidopsis thaliana | |
0.28 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant PDK mutant E238A | Arabidopsis thaliana | |
0.4 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant PDK mutant E238Q | Arabidopsis thaliana | |
3.5 | - |
pyruvate dehydrogenase | about, pH 7.5, 37°C, recombinant PDK mutant E238H | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate dehydrogenase | Arabidopsis thaliana | - |
ADP + phosphorylated pyruvate dehydrogenase | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SBJ1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type and mutant PDKs from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate dehydrogenase | - |
Arabidopsis thaliana | ADP + phosphorylated pyruvate dehydrogenase | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PDK | - |
Arabidopsis thaliana |
pyruvate dehydrogenase kinase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at, temperature-dependency of wild-type and mutant PDKs, overview | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant PDK mutant E238A | Arabidopsis thaliana | |
0.0005 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant PDK mutant E238H | Arabidopsis thaliana | |
0.07 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant PDK mutant L234A | Arabidopsis thaliana | |
0.28 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant PDK mutant H233A | Arabidopsis thaliana | |
0.39 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant PDK mutant L234H | Arabidopsis thaliana | |
0.55 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant wild-type PDK | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at, pH-dependency of wild-type and mutant PDKs, overview | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |