Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | domain-specific binding, isozymes PDK2 and PDK3 | Homo sapiens | |
acetyl-CoA | synergism with NADH | Homo sapiens | |
dihydrolipoyl transacetylase | stimulation rates in different buffers, stimulating domains for the isozyme PDK2 and PDK3 differ, overview | Homo sapiens | |
dihydrolipoyl transacetylase | lipoylation is required for binding, structural mutants stimulate less | Homo sapiens | |
NADH | domain-specific binding, isozymes PDK2 and PDK3 | Homo sapiens | |
NADH | synergism with acetyl-CoA | Homo sapiens | |
phosphate | isozyme PDK43, direct inhibition and elevation of the Km for ATP | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
isozymes PDK2 and PDK3, expression in Escherichia coli BL21(DE3) as His-tagged proteins | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 50-60% inhibition of isozyme PDK3, in presence of dihydrolipoyl transacetylase 70% | Homo sapiens | |
Dichloroacetate | inhibition of isozyme PDK3 is independent of dihydrolipoyl transacetylase, while isozyme PDK2 is more sensitive to inhibition when bound to it | Homo sapiens | |
additional information | carnitine, acetylcarnitine, malate, spermine, and calcium have no effect on isozyme PDK3 in presence of dihydrolipoyl transacetylase | Homo sapiens | |
thiamine diphosphate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
ATP | isozyme PDK2, 30°C, pH 7.3, MOPS-K+ buffer | Homo sapiens | |
0.006 | - |
ATP | isozyme PDK3, 30°C, pH 7.3, Tris-HEPES buffer | Homo sapiens | |
0.013 | - |
ATP | isozyme PDK3, 30°C, pH 7.3, MOPS-K+ buffer | Homo sapiens | |
0.023 | - |
ATP | isozyme PDK2, 30°C, pH 7.3, Tris-HEPES buffer | Homo sapiens | |
0.029 | - |
ATP | isozyme PDK3, 30°C, pH 7.3, phosphate buffer | Homo sapiens | |
0.04 | - |
ATP | isozyme PDK2, 30°C, pH 7.3, phosphate buffer | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45806 | - |
x * 45806, recombinant isozyme PDK2, detagged, amino acid determination | Homo sapiens |
46504 | - |
x * 46504, recombinant isozyme PDK3, detagged, amino acid determination | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (lipoamide)] | Homo sapiens | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozymes PDK2 and PDK3 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozymes PDK2 and PDK3 from Escherichia coli BL21(DE3) | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.44 | - |
isozyme PDK3, pH 7.3, 30°C | Homo sapiens |
0.69 | - |
isozyme PDK2, pH 7.3, 30°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (lipoamide)] | - |
Homo sapiens | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
ATP + [pyruvate dehydrogenase (lipoamide)] | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | Homo sapiens | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 45806, recombinant isozyme PDK2, detagged, amino acid determination | Homo sapiens |
? | x * 46504, recombinant isozyme PDK3, detagged, amino acid determination | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PDK | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
30 | - |
phosphate buffer | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.4 | assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Homo sapiens |