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Literature summary for 2.7.11.2 extracted from

  • Bowker-Kinley, M.; Popov, K.M.
    Evidence that pyruvate dehydrogenase kinase belongs to the ATPase/kinase superfamily (1999), Biochem. J., 344, 47-53.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type isozyme PDK2 and mutants in Escherichia coli as His-tagged enzymes Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D282A isozyme PDK2, site-directed mutagenesis, mutation of conserved amino acid, no activity, not able to bind ATP but the protein substrate Rattus norvegicus
G284A isozyme PDK2, site-directed mutagenesis, mutation of conserved amino acid, properties similar to wild-type Rattus norvegicus
G286A isozyme PDK2, site-directed mutagenesis, mutation of conserved amino acid, no activity, not able to bind ATP but the protein substrate Rattus norvegicus
G319A isozyme PDK2, site-directed mutagenesis, mutation of conserved amino acid, catalytically active, but very poor binding of ATP Rattus norvegicus
N247A isozyme PDK2, site-directed mutagenesis, mutation of conserved amino acid, no activity, not able to bind ATP but the protein substrate Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
ADP competitive to ATP; isozyme PDK2, wild-type and mutants G284A and G319A Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 ATP 37°C, recombinant isozyme PDK2 wild-type Rattus norvegicus
0.028
-
 ATP 37°C, recombinant mutant G284A of isozyme PDK2 Rattus norvegicus
1.5
-
 ATP 37°C, recombinant mutant G319A of isozyme PDK2 Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [pyruvate dehydrogenase (lipoamide)] Rattus norvegicus catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir

Organism

Organism UniProt Comment Textmining
Rattus norvegicus Q63065 isozyme PDK2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutants as His-tagged proteins from Escherichia coli BL21(DE3) Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate molecular modeling of the catalytic domain, structure Rattus norvegicus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [pyruvate dehydrogenase (lipoamide)]
-
 Rattus norvegicus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
ATP + [pyruvate dehydrogenase (lipoamide)] catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 Rattus norvegicus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir

Synonyms

Synonyms Comment Organism
More enzyme possibly belongs to the ATPase/kinase family Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2
-
 ADP 37°C, recombinant isozyme PDK2 wild-type Rattus norvegicus
0.3
-
 ADP 37°C, recombinant mutant G284A of isozyme PDK2 Rattus norvegicus
1.6
-
 ADP 37°C, recombinant mutant G319A of isozyme PDK2 Rattus norvegicus