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Literature summary for 2.7.11.2 extracted from
- Regulation of mammalian pyruvate dehydrogenase kinase (1997), FEBS Lett., 419, 197-200.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | involved in the regulation of enzyme activity regulating pyruvate dehydrogenase complex | Rattus norvegicus |  |
| dihydrolipoyl transacetylase | - |
Rattus norvegicus | |
| NADH | involved in the regulation of enzyme activity regulating pyruvate dehydrogenase complex | Rattus norvegicus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of isozyme PDK2 in Escherichia coli as His-tagged enzyme | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Dichloroacetate | potent and highly specific synthetic allosteric inhibitor mimicking pyruvate, inhibition mechanism | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [pyruvate dehydrogenase (lipoamide)] | Rattus norvegicus | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
isozyme PDK2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged PDK2 expressed in Escherichia coli | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [pyruvate dehydrogenase (lipoamide)] | - |
Rattus norvegicus | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
| ATP + [pyruvate dehydrogenase (lipoamide)] | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | Rattus norvegicus | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | dependent on | Rattus norvegicus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
Dichloroacetate | pH 7.4, 37°C | Rattus norvegicus | |
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Release 2023.1 (January 2023)
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