Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | competitive to ATP; inhibition only in the presence of monovalent cations; Mg2+ does not protect | Bos taurus | |
ATP | above 0.5 mM, substrate inhibition, only in the presence of K+, Mg2+ does not protect | Bos taurus | |
K+ | synergism with ADP | Bos taurus | |
Na+ | above 50 mM, alone and synergism with ADP | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the Km value for ADP depends on the presence and concentration of K+, effect of K+ on kinetic parameters | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | inhibits in presence of ADP | Bos taurus | |
Mg2+ | requirement | Bos taurus | |
Mg2+ | actual substrate: MgATP2- | Bos taurus | |
NH4+ | activation in the absence of ADP, inhibits in presence of ADP | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (lipoamide)] | Bos taurus | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (lipoamide)] | - |
Bos taurus | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
ATP + [pyruvate dehydrogenase (lipoamide)] | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | Bos taurus | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Ki value for ADP depends on the presence and concentration of monovalent cations, e.g. NH4+ or K+ | Bos taurus |