Cloned (Comment) | Organism |
---|---|
rabbit muscle phosphorylase kinase catalytic domain of catalytic subunit, expressed in Escherichia coli | Oryctolagus cuniculus |
Crystallization (Comment) | Organism |
---|---|
rabbit muscle phosphorylase kinase catalytic domain of catalytic subunit, i.e. Phkgammatrnc, in the presence of Mg-ATP, X-ray data | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
ATP | phosphorylase b, truncated form of phosphorylase kinase gamma subunit | Oryctolagus cuniculus | |
0.044 | 0.08 | phosphorylase b | holoenzyme | Oryctolagus cuniculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Oryctolagus cuniculus | |
Mg2+ | major role of Mg2+: cosubstrate in Mg2+-ATP complex | Oryctolagus cuniculus | |
phosphate | requirement, phosphate containing enzyme | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
catalytic subunit as expressed in Escherichia coli | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | - |
Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + phosphorylase b | cosubstrate: Mg-ATP complex | Oryctolagus cuniculus | ADP + phosphorylase a | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | alpha and beta subunits are regulatory subunits controlled by phosphorylation and proteolysis, Ca2+-sensitivity is conferred to delta subunit | Oryctolagus cuniculus |
More | the catalytic gamma subunit contains a kinase domain and a calmodulin binding domain | Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Oryctolagus cuniculus |