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Literature summary for 2.7.11.19 extracted from

  • Kilimann, M.W.; Heilmeyer, L.M.G.
    Multiple activities on phosphorylase kinase. 1. Characterization of three partial activities by their response to calcium ion, magnesium ion, pH, and ammonium chloride and effect of activation by phosphorylation and proteolysis (1982), Biochemistry, 21, 1727-1734.
    View publication on PubMed
Search for more literature for 2.7.11.19

Activating Compound

Activating Compound Comment Organism Structure
Catalytic subunit of cAMP-dependent protein kinase and beta Oryctolagus cuniculus
Catalytic subunit of cAMP-dependent protein kinase alpha Oryctolagus cuniculus
Catalytic subunit of cAMP-dependent protein kinase activation by enhancing vmax selectively for A2 activity Oryctolagus cuniculus
additional information phosphorylation by protein kinases Oryctolagus cuniculus
additional information the nonactivated enzyme is activated either by limited proteolysis Oryctolagus cuniculus
additional information three separate activities can be characterized by their response to Ca2+, Mg2+, NH4Cl and pH: A0, A1 and A2 Oryctolagus cuniculus
Trypsin proteolytic activation of nonactivated enzyme Oryctolagus cuniculus
Trypsin vmax enhancement of all three activities of the kinase: A0, A1 and A2 Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ inhibition in millimolar, activation in micromolar range Oryctolagus cuniculus
NH4Cl inhibits A1 and A2 activities by lowering of vmax, not A0 Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic properties of covalently modified and nonmodified phosphorylase kinase Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+-independent activity: A0 Oryctolagus cuniculus
Mg2+ requirement Oryctolagus cuniculus
Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Oryctolagus cuniculus
additional information three separate activities can be characterized by their response to Ca2+, Mg2+, NH4Cl and pH: A0, A1 and A2 Oryctolagus cuniculus
phosphate requirement, phosphate containing enzyme Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + phosphorylase b cosubstrate: Mg-ATP complex Oryctolagus cuniculus ADP + phosphorylase a
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 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pH-dependence of partial activities A1 and A2 Oryctolagus cuniculus