Literature summary for 2.7.11.18 extracted from

Lou, S.S.; Diz-Munoz, A.; Weiner, O.D.; Fletcher, D.A.; Theriot, J.A.
Myosin light chain kinase regulates cell polarization independently of membrane tension or Rho kinase (2015), J. Cell Biol., 209, 275-288.

Search for more literature for 2.7.11.18

Cloned(Commentary)

Cloned (Comment)	Organism
gene mylk5, expression analysis	Danio rerio

Protein Variants

Protein Variants	Comment	Organism
additional information	an antisense morpholino oligonucleotide is designed to induce mis-splicing of exon 9 of im:7148400 and early termination of the transcript before its kinase domain	Danio rerio

Inhibitors

Inhibitors	Comment	Organism	Structure
ML-7	-	Danio rerio

Organism

Organism	UniProt	Comment	Textmining
Danio rerio	F1QPV1	-	-
Danio rerio TL	F1QPV1	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
embryo	-	Danio rerio	-
keratocyte	from 2 d or 4 d postfertilization (dpf) embryos, im:7148400 is the only MLCK isozyme in keratocytes	Danio rerio	-

Synonyms

Synonyms	Comment	Organism
im:7148400	-	Danio rerio
MLCK	-	Danio rerio
mylk5	-	Danio rerio
myosin light chain kinase	-	Danio rerio

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Danio rerio

General Information

General Information	Comment	Organism
malfunction	decreasing MLCK activity in 4-dpf cells results in longer edge lifetimes and wider edges. Enzyme inhibition causes no change in the lateral propagation rate, but an increase in edge lifetime, which suggests that MLCK activity regulates edge size by controlling edge lifetime	Danio rerio
physiological function	keratocytes from 2 d postfertilization (dpf) embryos resemble canonical fanshaped keratocytes, but keratocytes from 4 dpf embryos often form multiple protrusions despite unchanged membrane tension. 4-dpf Cells have multiple protrusions because the protrusions are intrinsically small. The multiple-protrusion phenotype is primarily due to increased myosin light chain kinase (MLCK) expression. MLCK activity influences cell polarity by increasing myosin accumulation in lamellipodia, which locally decreases protrusion lifetime, limiting lamellipodial size and allowing for multiple protrusions to coexist within the context of membrane tension limiting protrusion globally, while Rho kinase regulates myosin accumulation at the cell rear and does not determine protrusion size. MLCK-specific mechanism for controlling cell polarity via regulation of myosin activity in protrusions, overview. Enzyme inhibition causes no change in the lateral propagation rate, but an increase in edge lifetime, which suggests that MLCK activity regulates edge size by controlling edge lifetime	Danio rerio

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Release 2023.1 (January 2023)