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Literature summary for 2.7.11.18 extracted from

  • Alcala, D.B.; Haldeman, B.D.; Brizendine, R.K.; Krenc, A.K.; Baker, J.E.; Rock, R.S.; Cremo, C.R.
    Myosin light chain kinase steady-state kinetics: comparison of smooth muscle myosin II and nonmuscle myosin IIB as substrates (2016), Cell Biochem. Funct., 34, 469-474.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.18

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin dependent on Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
additional information MLCK is inhibited as the ionic strength increases Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetic parameters of MLCK toward 4 different substrates: the 2-headed heavy meromyosin (HMM) soluble subfragments of both smooth muscle myosin II and non-muscle myosin IIB, each containing their respective smooth or non-muscle regulatory light chains, and the 2 types of free regulatory light chains. MLCK has an about 2fold higher kcat for both smooth muscle myosin II substrates compared with non-muscle myosin IIB substrates, whereas Km values are very similar Gallus gallus
0.0025
-
 [non-muscle heavy meromyosin IIB] pH 7.6, 25°C Gallus gallus
0.0026
-
 [smooth muscle heavy meromyosin] pH 7.6, 25°C Gallus gallus
0.0051
-
 [non-muscle regulatory light chain] pH 7.6, 25°C Gallus gallus
0.0072
-
 [smooth muscle regulatory light chain] pH 7.6, 25°C Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on Gallus gallus
Mg2+ required Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [myosin light chain] Gallus gallus non-muscle myosin II and preferred substrate smooth muscle myosin II ADP + [myosin light chain] phosphate
-
 ?
additional information Gallus gallus non-muscle myosin and smooth muscle myosin are the only known substrates of MLCK in vivo ?
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus P11799
-
-

Source Tissue

Source Tissue Comment Organism Textmining
gizzard
-
 Gallus gallus
-
additional information myosin light chain kinase is a ubiquitous Ca2+/calmodulin (CaM)-activated kinase found in smooth, cardiac, and skeletal muscle as well as in mammalian non-muscle cells Gallus gallus
-
smooth muscle
-
 Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [myosin light chain] non-muscle myosin II and preferred substrate smooth muscle myosin II Gallus gallus ADP + [myosin light chain] phosphate
-
 ?
ATP + [myosin light chain] phosphorylation of myosin on Ser19 of the regulatory light chain. Non-muscle myosin II regulatory light chain and preferred substrate smooth muscle myosin II regulatory light chain Gallus gallus ADP + [myosin light chain] phosphate
-
 ?
ATP + [non-muscle heavy meromyosin IIB]
-
 Gallus gallus ADP + [non-muscle heavy meromyosin IIB] phosphate
-
 ?
ATP + [non-muscle regulatory light chain]
-
 Gallus gallus ADP + [non-muscle regulatory light chain] phosphate
-
 ?
ATP + [smooth muscle heavy meromyosin]
-
 Gallus gallus ADP + [smooth muscle heavy meromyosin] phosphate
-
 ?
ATP + [smooth muscle regulatory light chain]
-
 Gallus gallus ADP + [smooth muscle regulatory light chain] phosphate
-
 ?
additional information non-muscle myosin and smooth muscle myosin are the only known substrates of MLCK in vivo Gallus gallus ?
-
 ?
additional information MLCK has a 2fold higher kcat for both smooth muscle myosin II substrates compared with non-muscle myosin IIB substrates, whereas Km values are very similar. Myosin light chain kinase has a 1.6fold and 1.5fold higher specificity (kcat/Km) for smooth versus non-muscle-free regulatory light chain and heavy meromyosin, respectively, suggesting that differences in specificity are dictated by regulatory light chain sequences. Three residues, Glu70, Gly71, and Ser74, are found to be surface exposed in the N-terminal half of the regulatory light chain, consistent with their importance in substrate recognition. Substrate recognition and specificity, overview Gallus gallus ?
-
 ?

Synonyms

Synonyms Comment Organism
MLCK
-
 Gallus gallus
MLCK-108
-
 Gallus gallus
MYLK
-
 Gallus gallus
myosin light chain kinase
-
 Gallus gallus
smooth muscle MLCK
-
 Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Gallus gallus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics: comparison of smooth muscle myosin II and non-muscle myosin IIB as substrates, overview. MLCK has an about 2fold higher kcat for both smooth muscle myosin II substrates compared with non-muscle myosin IIB substrates, whereas Km values are very similar Gallus gallus
8
-
 [non-muscle heavy meromyosin IIB] pH 7.6, 25°C Gallus gallus
12.6
-
 [non-muscle regulatory light chain] pH 7.6, 25°C Gallus gallus
12.8
-
 [smooth muscle heavy meromyosin] pH 7.6, 25°C Gallus gallus
28.6
-
 [smooth muscle regulatory light chain] pH 7.6, 25°C Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Gallus gallus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Gallus gallus

General Information

General Information Comment Organism
malfunction in knockout mice, the lack of MLCK-mediated phosphorylation of myosin in tracheal, bronchial, and gastrointestinal smooth muscle cannot be rescued by other kinases Gallus gallus
physiological function phosphorylation of myosin on S19 of the regulatory light chain (also called LC20) activates myosin ATPase activity in the presence of actin, which is necessary and sufficient for muscle contraction. All smooth muscles express two forms of myosin II, SMM (most abundant in adult tissues; includes 4 alternatively spliced variants derived from a single SMM heavy chain gene: 1A, 1B, 2A, and 2B) and NMM (IIA, IIB, and IIC minor). The smooth muscle enzyme prefers the smooth muscle myosin II regulatory light chain to the non-muscle myosin II. Non-muscle myosin IIB may participate in a physiologically relevant pathway for force maintenance in smooth muscle Gallus gallus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 additional information myosin light chain kinase has a 1.6fold and 1.5fold higher kcat/Km for smooth versus non-muscle-free regulatory light chain and heavy meromyosin, respectively Gallus gallus
2470
-
 [non-muscle regulatory light chain] pH 7.6, 25°C Gallus gallus
3200
-
 [non-muscle heavy meromyosin IIB] pH 7.6, 25°C Gallus gallus
3972
-
 [smooth muscle regulatory light chain] pH 7.6, 25°C Gallus gallus
4923
-
 [smooth muscle heavy meromyosin] pH 7.6, 25°C Gallus gallus