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Literature summary for 2.7.11.18 extracted from

  • McGinnis, L.A.; Lee, H.J.; Robinson, D.N.; Evans, J.P.
    MAPK3/1 (ERK1/2) and myosin light chain kinase in mammalian eggs affect myosin-II function and regulate the metaphase II state in a calcium- and zinc-dependent manner (2015), Biol. Reprod., 92, 146.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin required Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
ML-7
-
Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on Mus musculus
Mg2+ dependent on Mus musculus
Zn2+ dependent on Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [myosin light chain] Mus musculus
-
ADP + [myosin light chain] phosphate
-
?
ATP + [myosin light chain] Mus musculus CF-1
-
ADP + [myosin light chain] phosphate
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus Q3UIZ8
-
-
Mus musculus CF-1 Q3UIZ8
-
-

Source Tissue

Source Tissue Comment Organism Textmining
egg ovulated metaphase II eggs Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [myosin light chain]
-
Mus musculus ADP + [myosin light chain] phosphate
-
?
ATP + [myosin light chain]
-
Mus musculus CF-1 ADP + [myosin light chain] phosphate
-
?

Synonyms

Synonyms Comment Organism
MLCK
-
Mus musculus
myosin light chain kinase
-
Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Mus musculus

General Information

General Information Comment Organism
malfunction in meiosis I, MLCK inhibition and other myosin perturbations impair spindle migration and first polar body emission. Inhibition of the MEK1/2-MAPK pathway with U0126 leads to reduced levels of phosphorylated myosin-regulatory light chain (pMRLC) and causes a reduction in cortical tension, effects that are mimicked by treatment with the myosin light chain kinase (MLCK) inhibitor ML-7. MAPK or MLCK inhibition induces loss of normal cortical spindle localization or parthenogenetic egg activation. This parthenogenesis is dependent on cytosolic and extracellular calcium and can be rescued by hyperloading eggs with zinc, suggesting that these effects of inhibition of MLCK or the MAPK pathway are linked with dysregulation of ion homeostasis, overview Mus musculus
metabolism MAPK3/1 (ERK1/2, EC 2.7.11.24) and myosin light chain kinase in mammalian eggs affect myosin-II function and regulate the metaphase II state in a calcium- and zinc-dependent manner. Vertebrate eggs are arrested at metaphase of meiosis II, a state classically known as cytostatic factor arrest. Maintenance of this arrest until the time of fertilization and then fertilizationinduced exit from metaphase II are crucial for reproductive success, overview Mus musculus
physiological function MLCK phosphorylates myosin regulatory light chain (MRLC, also known as MYL9), which enables myosin-II organization into bipolar thick filaments Mus musculus