Literature summary for 2.7.11.18 extracted from

Clayburgh, D.R.; Rosen, S.; Witkowski, E.D.; Wang, F.; Blair, S.; Dudek, S.; Garcia, J.G.; Alverdy, J.C.; Turner, J.R.
A differentiation-dependent splice variant of myosin light chain kinase, MLCK1, regulates epithelial tight junction permeability (2004), J. Biol. Chem., 279, 55506-55513.

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Cloned(Commentary)

Cloned (Comment)	Organism
DNA sequence determination and analysis of splicing variant 1 of long endothelial enzyme, large scale expression of endothelial isozyme MLCK1 in Spodoptera frugiperda Sf9 cells via Baculovirus transfection system	Homo sapiens
DNA sequence determination and analysis of splicing variant 2 of long endothelial enzyme, large scale expression of endothelial isozyme MLCK2 in Spodoptera frugiperda Sf9 cells via Baculovirus transfection system	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	silencing of MLCK1 via specific siRNA increases transepithelial resistance in Caco-2 cells	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	isozyme MLCK1 is predominantly located in the perijunctional actomyosin ring in villus enterocytes	Homo sapiens	-	-
perinuclear space	isozyme MLCK2	Homo sapiens	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	the differentiation-dependent enzyme splice variant MLCK1 regulates epithelial tight junction permeability, the endothelial isozyme is responsible for Na+-glucose cotransport-induced tight junction regulation	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q15746	splicing variant 1, i.e. isozyme MLCK1; 2 endothelial isozymes MLCK1 and MLCK2	-
Homo sapiens	Q15746	splicing variant 2, i.e. isozyme MLCK2; 2 endothelial isozymes MLCK1 and MLCK2	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
Caco-2 cell	endothelial cell, 2 isozymes MLCK1 and MLCK2	Homo sapiens	-
endothelium	-	Homo sapiens	-
enterocyte	well differentiated cells, isozyme MLCK1	Homo sapiens	-
intestine	-	Homo sapiens	-
jejunum	-	Homo sapiens	-
additional information	expression profile of isozyme MLCK1	Homo sapiens	-
additional information	expression profile of isozymes MLCK1 and MLCK2	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the differentiation-dependent enzyme splice variant MLCK1 regulates epithelial tight junction permeability, the endothelial isozyme is responsible for Na+-glucose cotransport-induced tight junction regulation	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
MLCK	-	Homo sapiens

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Release 2023.1 (January 2023)