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Literature summary for 2.7.11.18 extracted from

  • Nakanishi, S.; Kakita, S.; Takahashi, I.; Kawahara, K.; Tsukada, E.; Sano, T.; Yamada, K.; Yoshida, M.; Kase, H.; Matsuda, Y.; Hashimoto, Y.; Nonomura, Y.
    Wortmannin, a microbial product inhibitor of myosin light chain kinase (1992), J. Biol. Chem., 267, 2157-2163.
    View publication on PubMed
Search for more literature for 2.7.11.18

Inhibitors

Inhibitors Comment Organism Structure
Wortmannin i.e. MS-54, IC50: 0.0019 mM, irreversible, highly selective, kinetics, high concentrations of ATP protect Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information in the presence of wortmannin Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + myosin light chain Gallus gallus
-
 ADP + myosin light chain phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
gizzard
-
 Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Lys-Lys-Arg-Pro-Gln-Arg-Ala-Thr-Ser-Asn-Val-Phe-Ser
-
 Gallus gallus ADP + ?
-
 ?
ATP + myosin light chain
-
 Gallus gallus ADP + myosin light chain phosphate
-
 ?
ATP + myosin light chain acceptor substrates are myosin light chains of smooth muscle myosin Gallus gallus ADP + myosin light chain phosphate
-
 ?
ATP + myosin light chain acceptor substrates are myosin light chains of smooth muscle Gallus gallus ADP + myosin light chain phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 assay at Gallus gallus

Cofactor

Cofactor Comment Organism Structure
Calmodulin requirement Gallus gallus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0019
-
 i.e. MS-54, IC50: 0.0019 mM, irreversible, highly selective, kinetics, high concentrations of ATP protect Gallus gallus Wortmannin