General Stability | Organism |
---|---|
glycerol and Tween 40 stabilize | Bos taurus |
unstable upon lyophilization | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cAMP-dependent protein kinase | phosphorylates light chain myosin kinase leading to decreased affinity from calmodulin | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000002 | - |
Calmodulin | 30°C, pH 6.8 | Bos taurus | |
0.02 | 0.027 | turkey gizzard myosin light chain | 30°C, pH 6.8 | Bos taurus | |
0.11 | - |
kemptamide | 30°C, pH 6.8 | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Bos taurus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Bos taurus | |
Mg2+ | Km-value: 2 mM | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
127000 | - |
sucrose density gradient centrifugation | Bos taurus |
152000 | - |
x * 152000, SDS-PAGE | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + myosin light chain | Bos taurus | - |
ADP + myosin light chain phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.8 | - |
myosin light chain, isolated | Bos taurus |
2.51 | - |
myosin light chain, in native myosin | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + kemptamide | - |
Bos taurus | ADP + ? | - |
? | |
ATP + myosin light chain | - |
Bos taurus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | acceptor substrates are myosin light chains of skeletal muscle | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | no substrate is casein | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | phosphorylation sites: Thr-residues in smooth muscle and pancreas myosin light chain | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | no substrate is histone 2 b | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | highly specific for regulatory or P-light-chain | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | highly specific for 18 kDa light chain | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | no substrate is synapsin | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | acceptor substrates are myosin light chains of smooth muscle | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | specific for 20 kDa light chain | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | no substrates are myelin basic protein, glycogen synthase, tubulin, microtubule-associated protein 2, kemptide and peptide pp60src | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | no substrate is phosphorylase b | Bos taurus | ADP + myosin light chain phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 152000, SDS-PAGE | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
More | - |
- |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | requirement | Bos taurus |