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Literature summary for 2.7.11.17 extracted from
- Biochemical characterization of Ca2+/calmodulin dependent protein kinase from Candida albicans (2003), Mol. Cell. Biochem., 252, 183-191.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | dependent on | Candida albicans |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DTNB | - |
Candida albicans | |
| KN-62 | a Ca2+/calmodulin-dependent enzyme inhibitor | Candida albicans | |
| additional information | no inhibition by H-7 and PKI | Candida albicans | |
| W-7 | a Ca2+/calmodulin antagonist | Candida albicans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Ka for calmodulin is 8.3 nM | Candida albicans | |
| 0.014 | - |
ATP | pH 7.0, 30°C | Candida albicans | |
| 0.02 | - |
syntide-2 | pH 7.0, 30°C | Candida albicans | |
| 0.063 | - |
Lys-Lys-Ala-Leu-Arg-Arg-Gln-Glu-Ala-Val-Asp-Ala-Leu | pH 7.0, 30°C | Candida albicans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Candida albicans | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | dependent on, activates | Candida albicans | |
| Mg2+ | dependent on | Candida albicans | |
| Mn2+ | partially stimulates | Candida albicans | |
| additional information | little or no effect by Ni2+, Ba2+, Zn2+, Cu2+, and Cd2+ | Candida albicans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69000 | - |
1 * 69000, SDS-PAGE | Candida albicans |
| 71000 | - |
gel filtration | Candida albicans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | - |
strain ATCC 3153, multifunctional enzyme | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | the enzyme performs Ca2+/calmodulin-dependent autophosphorylation at a Ser residue | Candida albicans |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 644fold from cytosol by ammonium sulfate fractionation, DEAE cellulose ion exchange chromatography, and gel filtration | Candida albicans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0035 | - |
purified enzyme | Candida albicans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + bovine serum albumin | low activity | Candida albicans | ADP + phosphorylated bovine serum albumin | - |
? | |
| ATP + casein | low activity | Candida albicans | ADP + phosphorylated casein | - |
? | |
| ATP + histone IIS | - |
Candida albicans | ADP + phosphorylated histone IIS | - |
? | |
| ATP + Kemptide | i.e. LRRASLG, low activity | Candida albicans | ADP + LRRAS(P)LG | - |
? | |
| ATP + KKALRRQETVDAL | i.e. autocamtide-2 | Candida albicans | ADP + KKALRRQET(P)VDAL | - |
? | |
| ATP + phosvitin | - |
Candida albicans | ADP + phosphorylated phosvitin | - |
? | |
| ATP + protamin | low activity | Candida albicans | ADP + phosphorylated protamin | - |
? | |
| ATP + syntide-2 | i.e. PLARTLSVAGLPGKK | Candida albicans | ADP + phosphorylated syntide-2 | - |
? | |
| additional information | the enzyme performs Ca2+/calmodulin-dependent autophosphorylation at a Ser residue | Candida albicans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 69000, SDS-PAGE | Candida albicans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ca2+/CaMPK | - |
Candida albicans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Candida albicans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
cytosolic extract, 60 min, inactivation | Candida albicans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Candida albicans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
broad range | Candida albicans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | dependent on | Candida albicans | |
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