Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.11.17 extracted from

  • D'Souza, J.S.; Johri, M.M.
    Purification and characterization of a Ca2+-dependent/calmodulin-stimulated protein kinase from moss chloronema cells (2003), J. Biosci., 28, 223-233.
    View publication on PubMed
Search for more literature for 2.7.11.17

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin dependent on, activates at 3-1000 nM Funaria hygrometrica
additional information the enzyme is activated by strictly Ca2+-dependent autophosphorylation Funaria hygrometrica

General Stability

General Stability Organism
activity of the purified enzyme is completely lost upon a freeze-thaw cycle Funaria hygrometrica

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on, activates, optimal at 0.05 mM Funaria hygrometrica
Mg2+
-
 Funaria hygrometrica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 x * 70000, SDS-PAGE Funaria hygrometrica

Organic Solvent Stability

Organic Solvent Comment Organism
n-propanol for extraction and as additive at 5% to the activity assay buffer Funaria hygrometrica

Organism

Organism UniProt Comment Textmining
Funaria hygrometrica
-
 a moss
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information the enzyme performs activating strictly Ca2+-dependent autophosphorylation which is influenced by substrates, overview Funaria hygrometrica

Purification (Commentary)

Purification (Comment) Organism
native enzyme from chloronemal cells 44fold to near homogeneity by ammoinum sulfate fractionation, DEAE-cellulose ion exchange chromatography, and calmodulin-agarose affinity chromatography in presence of CaCl2 Funaria hygrometrica

Source Tissue

Source Tissue Comment Organism Textmining
chloronema
-
 Funaria hygrometrica
-
protonema chloronemal cells Funaria hygrometrica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00069
-
 purified enzyme in presence of Ca2+ with substrate histone IIIS Funaria hygrometrica

Storage Stability

Storage Stability Organism
25°C, purified enzyme, increased activity at day 1 to 9, rapid loss within day 10, activity is completely lost upon a freeze-thaw cycle Funaria hygrometrica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + casein
-
 Funaria hygrometrica ADP + phosphorylated casein
-
 ?
ATP + histone IIA
-
 Funaria hygrometrica ADP + phosphorylated histone IIA
-
 ?
ATP + histone IIIS
-
 Funaria hygrometrica ADP + phosphorylated histone IIIS
-
 ?
ATP + histone VI
-
 Funaria hygrometrica ADP + phosphorylated histone VI
-
 ?
ATP + histone VIIS
-
 Funaria hygrometrica ADP + phosphorylated histone VIIS
-
 ?
ATP + histone VIS
-
 Funaria hygrometrica ADP + phosphorylated histone VIS
-
 ?
ATP + histone VS
-
 Funaria hygrometrica ADP + phosphorylated histone VS
-
 ?
additional information the enzyme prefers lysine-rich substrates, no activity with phosvitin and bovine serum albumin, the enzyme performs strictly Ca2+-dependent autophosphorylation which is influenced by substrates, overview Funaria hygrometrica ?
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Funaria hygrometrica

Synonyms

Synonyms Comment Organism
CCaMK
-
 Funaria hygrometrica
PK70
-
 Funaria hygrometrica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Funaria hygrometrica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Funaria hygrometrica

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Funaria hygrometrica