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Literature summary for 2.7.11.17 extracted from

  • Saneyoshi, T.; Kume, S.; Mikoshiba, K.
    Calcium/calmodulin-dependent protein kinase I in Xenopus laevis (2003), Comp. Biochem. Physiol. B, 134B, 499-507.
No PubMed abstract available
Search for more literature for 2.7.11.17

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin dependent on Xenopus laevis

Cloned(Commentary)

Cloned (Comment) Organism
isozyme CaM-KI LiKbeta, DNA and amino acid sequence determination and analysis, overexpression of a constitutively active CaM-KI LiKbeta mutant inhibits cell division in frog embryos, and causes severe changes in HeLa cell morphology Xenopus laevis
isozyme CaM-KIalpha, DNA and amino acid sequence determination and analysis, overexpression of a constitutively active CaM-KIalpha mutant inhibits cell division in frog embryos, and causes severe changes in HeLa cell morphology Xenopus laevis

Protein Variants

Protein Variants Comment Organism
K50E site-directed mutagenesis and truncation mutation constructing mutant K50E 1-295, the mutant isozyme CaM-KIalpha is constitutively active Xenopus laevis
K53E site-directed mutagenesis and truncation mutation constructing mutant K50E 1-297, the mutant isozyme CaM-KI LiKbeta is constitutively active Xenopus laevis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on Xenopus laevis
Mg2+
-
 Xenopus laevis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, amino acid sequence calculation, x * 45000, SDS-PAGE Xenopus laevis
43800
-
 x * 43800, amino acid sequence calculation, x * 45000, SDS-PAGE Xenopus laevis
45000
-
 x * 43000, amino acid sequence calculation, x * 45000, SDS-PAGE Xenopus laevis
45000
-
 x * 43800, amino acid sequence calculation, x * 45000, SDS-PAGE Xenopus laevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Xenopus laevis CaM-KI plays a role in cell structure regulation during early embryonic development ?
-
 ?

Organism

Organism UniProt Comment Textmining
Xenopus laevis Q8AYR3 CaM-KI; two isozymes CaM-KI or CaM-KI LiKbeta, and CaM-KIalpha
-
Xenopus laevis Q8AYR3 CaM-KIalpha; two isozymes CaM-KI or CaM-KI LiKbeta, and CaM-KIalpha
-

Source Tissue

Source Tissue Comment Organism Textmining
oocyte high expression level of isozyme CaM-KI LiKbeta in the neurula stage Xenopus laevis
-
oocyte the isozymes CaM-KIalpha is constitutively expressed through embryogenesis leading Xenopus laevis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + syntide-2 i.e. PLARTLSVAGLPGKK Xenopus laevis ADP + phosphorylated syntide-2
-
 ?
additional information CaM-KI plays a role in cell structure regulation during early embryonic development Xenopus laevis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, amino acid sequence calculation, x * 45000, SDS-PAGE Xenopus laevis
? x * 43800, amino acid sequence calculation, x * 45000, SDS-PAGE Xenopus laevis

Synonyms

Synonyms Comment Organism
calcium/calmodulin-dependent protein kinase I
-
 Xenopus laevis
calcium/calmodulin-dependent protein kinase Ialpha
-
 Xenopus laevis
CaM-KI
-
 Xenopus laevis
CaM-KI LiKbeta
-
 Xenopus laevis
CaM-KIalpha
-
 Xenopus laevis
XCaM-KI LiKbeta
-
 Xenopus laevis
XCaM-KIalpha
-
 Xenopus laevis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Xenopus laevis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Xenopus laevis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Xenopus laevis