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Literature summary for 2.7.11.17 extracted from
- A calmodulin-dependent protein kinase from lower eukaryote Physarum polycephalum (2005), Biochem. Biophys. Res. Commun., 328, 838-844.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | determination of the calmodulin binding regulatory domain | Physarum polycephalum |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cNA library construction and screening, DNA and amino acid sequence determination and analsis, expression in Escherichia coli strain BL21(DE3) with or without His-tag | Physarum polycephalum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Physarum polycephalum | |
| Mg2+ | - |
Physarum polycephalum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42519 | - |
x * 42519, amino acid sequence calculation | Physarum polycephalum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Physarum polycephalum | Q8WSQ3 | - |
- |
| Physarum polycephalum Ng-1 | Q8WSQ3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| non-tagged recombinant enzyme from Escherichia coli by CaM affinity chromatography and gel filtration, or His-tagged recombinant enzyme by nickel affinity chromatography | Physarum polycephalum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| plasmodium | - |
Physarum polycephalum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin II light chain | recombinant substrate from Physarum polycephalum, phosphorylation at Ser18, phosphorylation site analysis, no phosphorylation of substrate mutant S18A | Physarum polycephalum | ADP + phosphorylated myosin II light chain | - |
? | |
| ATP + myosin II light chain | recombinant substrate from Physarum polycephalum, phosphorylation at Ser18, phosphorylation site analysis, no phosphorylation of substrate mutant S18A | Physarum polycephalum Ng-1 | ADP + phosphorylated myosin II light chain | - |
? | |
| additional information | no activity with casein, bovine serum albumin, and light chains of various myosins from Dictyostelium, scallop, Mytilis, and chicken | Physarum polycephalum | ? | - |
? | |
| additional information | no activity with casein, bovine serum albumin, and light chains of various myosins from Dictyostelium, scallop, Mytilis, and chicken | Physarum polycephalum Ng-1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42519, amino acid sequence calculation | Physarum polycephalum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| calmodulin-dependent protein kinase | - |
Physarum polycephalum |
| CaM-dependent protein kinase | - |
Physarum polycephalum |
| CaMK | - |
Physarum polycephalum |
| More | the enzyme belongs to the family of calmodulin-dependent kinases, cf. EC 2.7.11.18 | Physarum polycephalum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Physarum polycephalum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Physarum polycephalum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Physarum polycephalum | |
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Release 2023.1 (January 2023)
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