Literature summary for 2.7.11.16 extracted from

Beyett, T.; Chen, Q.; Labudde, E.; Krampen, J.; Sharma, P.; Tesmer, J.
Characterization of a hyperphosphorylated variant of G protein-coupled receptor kinase 5 expressed in E. coli (2020), Protein Expr. Purif., 168, 105547 .

Search for more literature for 2.7.11.16

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 Rosetta cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
S500A	the mutant shows decreased activity compared to the wild type enzyme	Homo sapiens
T10A	the mutant shows increased activity compared to the wild type enzyme	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
CCG-215022	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0012	-	rhodopsin	recombinant mutant enzyme T10A, at pH 8.0 and 25°C	Homo sapiens
0.0018	-	rhodopsin	recombinant wild type enzyme, at pH 8.0 and 25°C	Homo sapiens
0.014	-	ATP	recombinant mutant enzyme T10A, at pH 8.0 and 25°C	Homo sapiens
0.014	-	ATP	recombinant wild type enzyme, at pH 8.0 and 25°C	Homo sapiens
0.02	-	ATP	recombinant mutant enzyme S500A, at pH 8.0 and 25°C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P34947	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	the enzyme is hyperphosphorylated	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
HiTrap S column chromatography and HiTrap Q column chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + rhodopsin	-	Homo sapiens	ADP + phosphorylated rhodopsin	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 70000, SDS-PAGE	Homo sapiens
monomer	1 * 68824, mass spectrometry	Homo sapiens

Synonyms

Synonyms	Comment	Organism
G protein-coupled receptor kinase 5	-	Homo sapiens
GRK5	-	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2.9	-	rhodopsin	recombinant wild type enzyme, at pH 8.0 and 25°C	Homo sapiens
4.1	-	ATP	recombinant mutant enzyme S500A, at pH 8.0 and 25°C	Homo sapiens
5.9	-	ATP	recombinant wild type enzyme, at pH 8.0 and 25°C	Homo sapiens
6.5	-	rhodopsin	recombinant mutant enzyme T10A, at pH 8.0 and 25°C	Homo sapiens
8.7	-	ATP	recombinant mutant enzyme T10A, at pH 8.0 and 25°C	Homo sapiens

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Release 2023.1 (January 2023)