Cloned (Comment) | Organism |
---|---|
expressed in Sf21 insect cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
mutant enzyme A486V crystallized in the presence of 5'-adenylyl beta,gamma-imidodiphosphate, sitting drop vapor diffusion method, at 4°C in 0.1 M sodium citrate, pH 5.0-6.5, 15-27.5% (w/v) polyethylene glycol 3350 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A142V | the mutation is associated with hypertension | Homo sapiens |
A486V | the mutation is associated with hypertension. There is an increased rate of autophosphorylation of a number of residues in the mutant relative to the wild type enzyme | Homo sapiens |
R65L | the mutation is associated with hypertension | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [G-protein-coupled receptor] | Homo sapiens | - |
ADP + [G-protein-coupled receptor]phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P32298 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography, HiTrap SP column chromatography, HiTrap heparin column chromatography and Superdex 75 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [G-protein-coupled receptor] | - |
Homo sapiens | ADP + [G-protein-coupled receptor]phosphate | - |
? | |
additional information | autophosphorylation of wild type isoform GRK4alpha is required for full kinase activity | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G protein-coupled receptor kinase 4 | - |
Homo sapiens |
GRK4 | - |
Homo sapiens |