Literature summary for 2.7.11.15 extracted from

Mayer, G.; Wulffen, B.; Huber, C.; Brockmann, J.; Flicke, B.; Neumann, L.; Hafenbradl, D.; Klebl, B.M.; Lohse, M.J.; Krasel, C.; Blind, M.
An RNA molecule that specifically inhibits G-protein-coupled receptor kinase 2 in vitro (2008), RNA, 14, 524-534.

Search for more literature for 2.7.11.15

Cloned(Commentary)

Cloned (Comment)	Organism
expression of GST-tagged GRK2 and GRK2 C-terminal or N-terminal domains in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
RNA aptamer C13	development and synthesis of a highly specific RNA aptamer C13 that potently inhibits GRK2 by binding to it kinase domain, binding kinetics, inhibitor secondary structure, overview	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant GST-tagged GRK2 and GRK2 C-terminal or N-terminal domains from insect Sf9 cells by ion exchange and heparin affinity chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	GRK2 also phosphorylates rhodopsin, cf. EC 2.7.11.14	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
G-protein-coupled receptor kinase 2	-	Homo sapiens
GRK2	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at room temperature	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0000041	-	inhibition of GRK2, kinetics, overview	Homo sapiens	RNA aptamer C13

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Release 2023.1 (January 2023)