Cloned (Comment) | Organism |
---|---|
expression of GRK2 and GRK3 in cardiac myocytes via adenoviral transfection, overview | Rattus norvegicus |
expression of minigene encoding peptide inhibitors of GRK2, the C-terminal fragments of GRK2, i.e. AdGRK2, and AdGRK2ct, using adenovirus infection of cardiac myocyte, with effects on rod-shaped morphology of cardiac myocytes, overview | Rattus norvegicus |
expression of minigene encoding peptide inhibitors of GRK3, the C-terminal fragments of GRK3, i.e. AdGRK3, or AdGRK3ct, using adenovirus infection of cardiac myocyte, with effects on rod-shaped morphology of cardiac myocytes, overview | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | subcellular distribution analysis of GRK2 and GRK3 | Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79000 | - |
x * 79000, GRK3, SDS-PAGE, x * 81000, GRK2, SDS-PAGE | Rattus norvegicus |
81000 | - |
x * 79000, GRK3, SDS-PAGE, x * 81000, GRK2, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha1 beta-adrenergic receptor | Rattus norvegicus | substrate specificities of GRK2 and GRK3 in cardiac myocytes, overview | ADP + phosphorylated alpha1 beta-adrenergic receptor | - |
? | |
ATP + alpha1-adrenergic receptor | Rattus norvegicus | - |
ADP + alpha1-adrenergic receptor phosphate | - |
? | |
ATP + beta1-adrenergic receptor | Rattus norvegicus | - |
ADP + beta1-adrenergic receptor phosphate | - |
? | |
ATP + rhodopsin | Rattus norvegicus | - |
ADP + phosphorylated rhodopsin | - |
? | |
additional information | Rattus norvegicus | GRK2 substrate specificity in adult rat cardiac myocytes, overview. GRK2 completely lacks the capacity to attenuate phenylephrine-stimulated IP generation | ? | - |
? | |
additional information | Rattus norvegicus | GRK3 substrate specificity in adult rat cardiac myocytes, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Wistar rats | - |
Rattus norvegicus | P26819 | GRK2; male Wistar rats | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cardiac myocyte | - |
Rattus norvegicus | - |
cardiomyocyte | - |
Rattus norvegicus | - |
heart | - |
Rattus norvegicus | - |
additional information | quantitative enzyme level determination | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
quantitative enzyme level determination | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha1 beta-adrenergic receptor | - |
Rattus norvegicus | ADP + phosphorylated alpha1 beta-adrenergic receptor | - |
? | |
ATP + alpha1 beta-adrenergic receptor | substrate specificities of GRK2 and GRK3 in cardiac myocytes, overview | Rattus norvegicus | ADP + phosphorylated alpha1 beta-adrenergic receptor | - |
? | |
ATP + alpha1-adrenergic receptor | - |
Rattus norvegicus | ADP + alpha1-adrenergic receptor phosphate | - |
? | |
ATP + beta1-adrenergic receptor | - |
Rattus norvegicus | ADP + beta1-adrenergic receptor phosphate | - |
? | |
ATP + rhodopsin | - |
Rattus norvegicus | ADP + phosphorylated rhodopsin | - |
? | |
ATP + rhodopsin | substrate in dark-adapted, urea-stripped rod outer segment membranes | Rattus norvegicus | ADP + phosphorylated rhodopsin | - |
? | |
additional information | GRK2 substrate specificity in adult rat cardiac myocytes, overview. GRK2 completely lacks the capacity to attenuate phenylephrine-stimulated IP generation | Rattus norvegicus | ? | - |
? | |
additional information | GRK3 substrate specificity in adult rat cardiac myocytes, overview | Rattus norvegicus | ? | - |
? | |
additional information | GRK2 substrate specificity, overview. GRK2 also shows activity with rhodopsin as substrate, cf. EC 2.7.11.14 | Rattus norvegicus | ? | - |
? | |
additional information | GRK3 substrate specificity, overview. GRK3 also shows activity with rhodopsin as substrate, cf. EC 2.7.11.14 | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 79000, GRK3, SDS-PAGE, x * 81000, GRK2, SDS-PAGE | Rattus norvegicus |
More | GRK2 consists of three domains: an aminoterminal RGS homology domain, a central protein kinase domain, and a carboxyl-terminal pleckstrin homology, PH, domain | Rattus norvegicus |
More | GRK3 consists of three domains: an aminoterminal RGS homology domain, a central protein kinase domain, and a carboxyl-terminal pleckstrin homology, PH, domain | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
G protein-coupled receptor kinase-2 | - |
Rattus norvegicus |
G protein-coupled receptor kinase-3 | - |
Rattus norvegicus |
GRK2 | - |
Rattus norvegicus |
GRK3 | - |
Rattus norvegicus |
More | GRK2 and GRK3 constitute a subfamily among the different GRK isoforms | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |