Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant GRK2 in C3H10T1/2 cells, transient expression in HEK-293 cells, expression of GRK2, but not catalytically inactive GRK2, synergizes with active Smoothened to mediate Gli-dependent transcription | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
K220R | inactive GRK2 mutant | Bos taurus |
additional information | knockdown of endogenous GRK2 by short hairpin RNA significantly reduces signaling in response to the Smoothened agonist SAG and also inhibits signaling induced by an oncogenic Smoothened mutant, Smo M2 | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Smoothened protein | Bos taurus | GRK2 promotes smoothened signal transduction involved in regulation of cellular proliferation and differentiation through activation of the transcription factor Gli, overview | ADP + phosphorylated Smoothened | - |
? | |
additional information | Bos taurus | GRK2 regulates 7TM G-protein-coupled receptor activity, GRK2 promotes the association between active Smoothened and beta-arrestin 2, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Smoothened protein | GRK2 promotes smoothened signal transduction involved in regulation of cellular proliferation and differentiation through activation of the transcription factor Gli, overview | Bos taurus | ADP + phosphorylated Smoothened | - |
? | |
ATP + Smoothened protein | a seven-transmembrane signaling protein | Bos taurus | ADP + phopshorylated Smoothened | - |
? | |
additional information | GRK2 regulates 7TM G-protein-coupled receptor activity, GRK2 promotes the association between active Smoothened and beta-arrestin 2, overview | Bos taurus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G protein-coupled receptor kinase 2 | - |
Bos taurus |
GRK2 | - |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bos taurus |