Cloned (Comment) | Organism |
---|---|
cytosolic expression of wild-type and mutant GRK2 in HEK-293 cells, co-expression with the beta-adrenergic receptor | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutation of the clathrin-binding motif of GRK2 results in a dominant negative form of GRK2 without affecting kinase activity itself and the ability to interact with beta2-adrenergic receptor, no translocation of beta-adrenergic receptors to endocytic vesicles as well as GRK2 translocation to the plasma membrane with the dominant-negative mutant and mutant GRK2-5A, overview | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | the wild-type GRK2 is translocated to the plasma membrane | Rattus norvegicus | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-adrenergic receptor | Rattus norvegicus | phosphorylation and internalization of the receptor requires clathrin, GRK2 specifically phosphorylates the activated form of the receptor that promotes the translocation of beta-arrestins to the plasma membrane, overview | ADP + phosphorylated beta-adrenergic receptor | - |
? | |
ATP + rhodopsin | Rattus norvegicus | light-activated rhodopsin | ADP + phosphorylated rhodopsin | - |
? | |
additional information | Rattus norvegicus | GRK2 activity leads to translocation of parts of beta-adrenergic receptors to endocytic vesicles | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-adrenergic receptor | phosphorylation and internalization of the receptor requires clathrin, GRK2 specifically phosphorylates the activated form of the receptor that promotes the translocation of beta-arrestins to the plasma membrane, overview | Rattus norvegicus | ADP + phosphorylated beta-adrenergic receptor | - |
? | |
ATP + beta-adrenergic receptor | urea-treated rod outer segments as substrate | Rattus norvegicus | ADP + phosphorylated beta-adrenergic receptor | - |
? | |
ATP + rhodopsin | light-activated rhodopsin | Rattus norvegicus | ADP + phosphorylated rhodopsin | - |
? | |
additional information | GRK2 activity leads to translocation of parts of beta-adrenergic receptors to endocytic vesicles | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G protein-coupled receptor kinase 2 | - |
Rattus norvegicus |
GRK2 | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |