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Literature summary for 2.7.11.15 extracted from

  • Eichmann, T.; Lorenz, K.; Hoffmann, M.; Brockmann, J.; Krasel, C.; Lohse, M.J.; Quitterer, U.
    The amino-terminal domain of G-protein-coupled receptor kinase 2 is a regulatory Gbeta gamma binding site (2003), J. Biol. Chem., 278, 8052-8057.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
G protein Gbetagamma subunits required for activity, 20fold stimulation with substrate rhodopsin, 2 regulatory Gbetagamma binding sites: one N-terminal domain of GRK2, and one C-terminal within the pleckstrin homology domain, both sites are functionally different, overview Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-tagged wild-type and truncation mutant GRK2s in Escherichia coli, expression of wild-type GRK2 in Spodoptera frugiperda Sf9 cells using the baculovirus infection system, expression of wild-type and truncation mutant GRK2s in HEK-293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of deletion mutants comprising residues 1-485, 1-185, 561-689, 1-53, 1-200, or 54-185, analysis of activity with rhodopsin and binding of G protein betagamma subunits, overview Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information deletion mutants of the enzyme, comprising parts of the C-terminus or the N-terminus, are able to inhibit the full-length wild-type enzyme, overview Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged wild-type and truncation mutant GRK2s from Escherichia coli by glutathione affinity chromatography, recombinant wild-type GRK2 from Sf9 insect cells by heparin affinity chromatography and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + rhodopsin
-
Homo sapiens ADP + phosphorylated rhodopsin
-
?

Synonyms

Synonyms Comment Organism
G protein-coupled receptor kinase 2
-
Homo sapiens
GRK2
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens