Cloned (Comment) | Organism |
---|---|
expression of GRK2 in Spodoptera frugiperda Sf9 cells | Homo sapiens |
General Stability | Organism |
---|---|
glycerol stabilizes | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microtubule | - |
Homo sapiens | 5874 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GRK2 from insect Sf9 cells by ammonium sulfate fractionation, hydrophobic interaction and heparin affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dephospho-beta-tubulin | purified substrate from porcine brain or human GST-tagged protein recombinantly expressed in Escherichia coli, phosphorylation of betaI- and betaIII-tubulin at Thr409, Ser420, and, in betaIII-tubulin, also at Ser420 of the C-terminal outer surface of the substrate protein | Homo sapiens | ADP + phosphorylated beta-tubulin | - |
? | |
ATP + phosducin | - |
Homo sapiens | ADP + phosphorylated phosducin | - |
? | |
ATP + synuclein | - |
Homo sapiens | ADP + phosphorylated synuclein | - |
? | |
additional information | GRK2 prefers acidic protein sequences for phosphorylation | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G-protein-coupled receptor kinase 2 | - |
Homo sapiens |
GRK2 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |